A novel polyelectrolytical complex membrane between chitosan and carboxymethyl chitosan has been prepared and the primary structure characterization has been performed. Meantime, the lysozyme adsorption capacities on these complex membranes with various chitosan/carboxymethyl chitosan compositions have been tested. The results showed that the complex membranes had significant adsorption capacity to lysozyme and the adsorption capacity increased with the increase of carboxymethyl chitosan content in the membrane. When carboxymethyl chitosan content in the membrane is 40%, the lysozyme adsorption capacity can reach as high as 92 mg/g.

Key words: membrane chromatography, blend, macroporous membrane, protein, adsorption, crosslinking