Seventeen novel 6-aryl-3-[2-(4-chlorophenoxymethylbenzimidazole)-1-methylene]-1,2,4-tria- zolo[3,4-b]-1,3,4-thiadiazoles 7 were synthesized by the condensation of 4-amino-5-[2-(4-chlorophenoxy- methylbenzimidazole)-1-methylene]-3-mercapto-1,2,4-triazole (6) with various aromatic acids in the prese- nce of phosphorous oxychloride. The structures of the intermediate 6 and the target compounds 7 were characterized by IR, ¹H NMR techniques and elemental analysis. Preliminary bioassay indicated that the target compounds 7a and 7b exhibited higher inhibitory activity to EcMetAP1 with the inhibition of 87.26% and 82.62% respectively, compounds 7f, 7h and 7l showed moderate activity at the concentration of 20 μg/mL. The compounds 7a～7q had obvious inhibition to the cell devision cycle 25B phosphatase at 20 μg/mL and the compounds 7b, 7g and 7i still had higher inhibition even at 5 μg/mL with the inhibition of 98.76%, 83.87% and 90.57% respectively. The results of screening for fungicidal, insecticidal, herbicidal and plant growth regulating activities indicated that the tested target compounds 7d～7e, 7h～7m and 7o～7q all showed no activities.

Key words: triazolo-thiadiazoles, synthesis, characterization, bioactivities