In vitro Nitration and Oxidation of Recombinant Human Interferon α2b by Peroxynitrite

Acta Chimica Sinica ›› 2010, Vol. 68 ›› Issue (19): 2024-2028. Previous Articles

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过氧亚硝基阴离子对重组人干扰素α2b的氧化硝化修饰特征

赵广荣*，田莉莉，马琼，乔斌

(天津大学化工学院制药工程系系统生物工程教育部重点实验室天津 300072)

投稿日期:2010-02-03 修回日期:2010-05-24 发布日期:2010-06-09
通讯作者: 赵广荣 E-mail:grzhao@tju.edu.cn
基金资助:
国家自然科学基金项目;天津市自然科学基金资助

In vitro Nitration and Oxidation of Recombinant Human Interferon α2b by Peroxynitrite

ZHAO Guang-Rong, TIAN Li-Li, MA Qiong, QIAO Bin

(Key Laboratory of Systems Bioengineering, Ministry of Education, Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072)

Received:2010-02-03 Revised:2010-05-24 Published:2010-06-09
Contact: Guang-Rong ZHANG E-mail:grzhao@tju.edu.cn
Supported by:
;Tianjin Natural Science Foundation

Abstract

In vitro nitration/oxidation characterization of recombinant human interferon α2b (rhIFN α2b) induced by peroxynitrite (ONOO^－) was investigated. The modified peptide fragments of rhIFN α2b were analyzed, and the oxidized and nitrated amino acids were identified by HPLC-MS/MS. The results showed that Met16 of the helix A in rhIFN α2b was oxidized, and Tyr135 of the DE loop and Trp140 of the helix E were nitrated.

Key words: recombinant human interferon α2b, peroxynitrite, oxidation, nitration, amino acid modification

Cite this article

ZHAO Guang-Rong, TIAN Li-Li, MA Qiong, QIAO Bin. In vitro Nitration and Oxidation of Recombinant Human Interferon α2b by Peroxynitrite[J]. Acta Chimica Sinica, 2010, 68(19): 2024-2028.

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过氧亚硝基阴离子对重组人干扰素α2b的氧化硝化修饰特征

In vitro Nitration and Oxidation of Recombinant Human Interferon α2b by Peroxynitrite

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