Microcantilever Biosensor on the Biotin-avidin Interaction

Acta Chimica Sinica ›› 2011, Vol. 69 ›› Issue (02): 243-246. Previous Articles Next Articles

Note

微悬臂生物传感器检测生物素-亲和素相互作用

张慧勇¹^{,2，潘宏青¹^{,2，唐纪琳*^{,1，张柏林*^,1}}}

(¹中国科学院长春应用化学研究所电分析化学国家重点实验室长春 130022)
(²中国科学院研究生院北京 100049)

投稿日期:2010-03-09 修回日期:2010-06-29 发布日期:2010-08-16
通讯作者: 张慧勇 E-mail:beanphq@ciac.jl.cn

Microcantilever Biosensor on the Biotin-avidin Interaction

Zhang Huiyong¹^{,2 Pan Hongqing¹^{,2 Tang Jilin*^{,1 Zhang Bailin*^,1}}}

(¹State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022)
(²Graduate University of Chinese Academy of Sciences, Beijing 100049)

Received:2010-03-09 Revised:2010-06-29 Published:2010-08-16

Abstract

The dynamic interaction process of biotin-avidin was investigated by biotin covalently modified microcantilever sensor in real-time in a flowing cell. The specific interaction was confirmed by the evidence that the avidin layer can be mechanically removed with atomic force microscopy nanolithography. The deflection of the modified cantilever increased with concentration of avidin. Microcantilever biosensor provides a good method to explore novel label-free technology and monitor various interactions between biomolecules.

Key words: microcantilever, biotin, avidin, interaction

Cite this article

ZHANG Hui-Yong, PAN Hong-Qing, TANG Ji-Lin, ZHANG Bai-Lin. Microcantilever Biosensor on the Biotin-avidin Interaction[J]. Acta Chimica Sinica, 2011, 69(02): 243-246.

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微悬臂生物传感器检测生物素-亲和素相互作用

Microcantilever Biosensor on the Biotin-avidin Interaction

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