Acta Chimica Sinica ›› 2011, Vol. 69 ›› Issue (18): 2196-2200. Previous Articles    

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非标记夹心式电化学可卡因适体传感器的研究

上官莉,漆红兰*,凌晨   

  1. (陕西省生命分析化学重点实验室 陕西师范大学化学与材料科学学院 西安 710062)
  • 投稿日期:2010-11-30 修回日期:2011-04-15 发布日期:2011-05-23
  • 通讯作者: 漆红兰 E-mail:honglanqi@snnu.edu.cn
  • 基金资助:

    肝癌标示物电化学微阵列传感器的研究;电化学糖生物传感器的构建及其在肿瘤标示物分析中的应用

Label-free and Sandwich Aptamer-based Electrochemical Biosensor for the Determination of Cocaine

SHANG Guan-Li, QI Hong-Lan, LING Chen   

  1. (Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, Xian 710062)
  • Received:2010-11-30 Revised:2011-04-15 Published:2011-05-23
  • Contact: Hong-Lan QI E-mail:honglanqi@snnu.edu.cn
  • Supported by:

    Electrochemical microarray for the determination of marker of liver cancer

A label-free and sandwich aptamer-based electrochemical biosensor for simple and sensitive determination of cocaine was developed based on double-strand nucleotide aptamer. Capture ss-DNA was immobilized on gold surfaces by means of self-assembly technique to fabricate cocaine aptamer-based biosensor. In the presence of target cocaine and detected aptamer probe, two fragments folded with cocaine and formed a sandwich complex on the electrode surface. Based on the difference binding ability of [Ru(NH3)6]3+ to ss-DNA and sandwich complex, a simple and highly sensitive electrochemical method was developed for the determination of cocaine by detecting the charge of [Ru(NH3)6]3+ adsorbed on the electrode surface by chronocoulometry. Under the optimized conditions, the charge response was linear with the concentration of cocaine from 1 mmol/L to 50 mmol/L. The detection limit was 0.1 mmol/L. The developed method allows the detection of cocaine in a serum sample with recovery in 96.4%~104%. This design strategy is easy to generalize for any aptamer without prior knowledge of its secondary or tertiary structure, and would be used as a simple, sensitive and general tool for biomolecule detection.

Key words: label-free, electrochemistry, sensor, aptamer, cocaine

CLC Number: