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Acta Chimica Sinica ›› 2008, Vol. 66 ›› Issue (24): 2705-2711. Previous Articles     Next Articles

Original Articles

配合物[Co2(EGTB)Cl2]•(BF4)2•5H2O与DNA相互作用的研究

朱 莉*,a,b 彭 斌b 凌 友b 林原斌*,a

  

  1. (a湘潭大学化学学院 湘潭 411105)
    (b湖南科技大学化学化工学院 分子构效关系湖南省普通高等学校重点实验室 湘潭 411201)

  • 投稿日期:2008-07-16 修回日期:2008-10-27 发布日期:2008-12-28
  • 通讯作者: 朱莉

Interaction of Complex [Co2(EGTB)Cl2]•(BF4)2•5H2O with DNA

ZHU, Li *,a,b PENG, Bin b LING, You b LIN, Yuan-Bin *,a   

  1. (a Chemistry Institute, Xiangtan University, Xiangtan 411105)
    (b Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, China)
  • Received:2008-07-16 Revised:2008-10-27 Published:2008-12-28
  • Contact: ZHU, Li

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The interaction of Co(II) complex [Co2(EGTB)Cl2]•(BF4)2•5H2O with DNA has been investigated by electronic absorption spectroscopy, fluorescence spectroscopy, viscosity and gel electrophoresis measurements. The apparent binding constants of the complex with DNA was obtained at pH＝7.2. The results indicated that the Co(II) complex could cleave pBR322DNA effectively at physiological pH and temperature, and also increase the viscosity of DNA. The emission intensity was quenched as the complex was added to the EB-DNA system. The results suggest that the interaction of Co(II) complex with DNA belong to both partial intercalation and static electricity attraction mode.

Key words: Co(II) complex, synthesis, DNA, partial intercalation, electricity attraction, pBR322DNA