Interaction of Agarose-based Oligosaccharides with Agglutinin RCA120 Using Glycochip Technology

Acta Chimica Sinica ›› 2011, Vol. 69 ›› Issue (08): 955-959. Previous Articles Next Articles

Full Papers

糖芯片技术研究琼胶寡糖与凝集素RCA₁₂₀相互作用

王玉峰¹，于广利*^,1,2，韩章润²，吴建东²，赵小亮²，柴文刚^2,3

(¹海洋药物教育部重点实验室中国海洋大学青岛 266003)
(²山东省糖科学与糖工程重点实验室青岛 266003)
(³ Glycosciences Laboratory, Faculty of Medicine, Imperial College London, Harrow, Middlesex HA1 3UJ, United Kingdom)

投稿日期:2010-10-20 修回日期:2010-11-25 发布日期:2010-12-13
通讯作者: 王玉峰 E-mail:wy511813@gmail.com
基金资助:
国际科技合作专项;国家海洋局公益性行业专项;国家自然科学基金;长江学者和创新团队发展计划

Interaction of Agarose-based Oligosaccharides with Agglutinin RCA₁₂₀ Using Glycochip Technology

Wang Yufeng¹ Yu Guangli*^,1,2 Han Zhangrun² Wu Jiandong² Zhao Xiaoliang² Chai Wengang^2,3

(¹Key Laboratory of Marine Drugs, Ministry of Education, Ocean University of China, Qingdao 266003)
(²Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, Qingdao 266003)
(³Glycosciences Laboratory, Faculty of Medicine, Imperial College London, Harrow, Middlesex HA1 3UJ, United Kingdom)

Received:2010-10-20 Revised:2010-11-25 Published:2010-12-13
Contact: Yufeng Wang E-mail:wy511813@gmail.com

Nine oligosaccharides were obtained from agarose by chemical and enzymatic degradation and gel filtration chromatography. All oligosaccharides were linked to 1,2-dihexadecyl-sn-glycero-3-phospho- ethanolamine (DHPE) by a reductive animation reaction to acquire neoglycolipids. Compared with standard oligosaccharides (LNnT and LNT), the affinity of agaro-oligosaccharides to RCA₁₂₀ was studied using glycochip technique. We firstly found the higher affinity of oligosaccharides with structure of Galβ1,4AnG-R than that of Galβ1,4GlcNAc-R to RCA₁₂₀ compared with LNnT, and the affinity have no relation with degree of polymerization.

Key words: agaro-oligosaccharide, neoglycolipid, glycochip, RCA₁₂₀, interaction

Cite this article

WANG Yu-Feng, YU Guang-Li, HAN Zhang-Run, WU Jian-Dong, ZHAO Xiao-Liang, CHAI Wen-Gang. Interaction of Agarose-based Oligosaccharides with Agglutinin RCA₁₂₀ Using Glycochip Technology[J]. Acta Chimica Sinica, 2011, 69(08): 955-959.

Export EndNote|Reference Manager|ProCite|BibTeX|RefWorks

[1]	Lei Yang, Jiaoyang Ge, Fangli Wang, Wangyang Wu, Zongxiang Zheng, Hongtao Cao, Zhou Wang, Xueqin Ran, Linhai Xie. A Theoretical Study on the Effective Reduction of Internal Reorganization Energy Based on the Macrocyclic Structure of Fluorene [J]. Acta Chimica Sinica, 2023, 81(6): 613-619.
[2]	Mingrui Pu, Feng He. Chlorine-Mediated Organic Photovoltaic Materials^★ [J]. Acta Chimica Sinica, 2023, 81(11): 1541-1550.
[3]	Wen Liu, Yujie Wang, Huiqin Yang, Chengjie Li, Na Wu, Yang Yan. The Preparation of Carbon Nanotubes/Reduced Graphene Oxide Current Collector by Non-covalent Induction of Ionic Liquid for Sodium Metal Anode [J]. Acta Chimica Sinica, 2023, 81(10): 1379-1386.
[4]	Yahui Jia, Chunsheng Li, Zhongzhen Xu, Wei Liu, Daowei Gao, Guozhu Chen. The SMSI of Pt-TiO₂ During the Crystalline Phase Transformation and Its Effect on CO Oxidation Performance [J]. Acta Chimica Sinica, 2022, 80(9): 1289-1298.
[5]	Shimin Zhu, Xin Huang, Xie Han, Simin Liu. Recognition and Luminescence Properties of N^C^N Pt(II) Complexes with Macrocyclic Host Cucurbit[10]uril [J]. Acta Chimica Sinica, 2022, 80(8): 1066-1070.
[6]	Zhihao Qi, Fujie Gao, Changkai Zhou, Yu Zeng, Qiang Wu, Lijun Yang, Xizhang Wang, Zheng Hu. Ruthenium Nanoparticles Anchored on Nitrogen-Doped Carbon Nanocages for Fischer-Tropsch Synthesis [J]. Acta Chimica Sinica, 2022, 80(8): 1100-1105.
[7]	Zhiliang Gao, Mengqi Li, Jingcheng Hao, Jiwei Cui. Tuning the Mechanical Properties of Colloid Particles for Drug Delivery [J]. Acta Chimica Sinica, 2022, 80(7): 1010-1020.
[8]	Yuwan Chen, Wen Zhou, Xinwei Li, Kaiguang Yang, Zhen Liang, Lihua Zhang, Yukui Zhang. Research Progress of Protein-Protein Interaction Based on Liquid Chromatography Mass Spectrometry^※ [J]. Acta Chimica Sinica, 2022, 80(6): 817-826.
[9]	Chuanzhi Liu, Fen Li, Jingjing Wang, Xiaolu Zhao, Tingmei Zhang, Xin Huang, Mengli Wu, Zhiyuan Hu, Xinming Liu, Zhanting Li. Self-assembly of Supramolecular Planar Macrocycle Driven by Intermolecular Halogen Bonding [J]. Acta Chimica Sinica, 2022, 80(10): 1365-1368.
[10]	Bo Yang, Yongli Zhang, Hongguang Guo. Multi-spectroscopic Investigation on Mechanism of Binding Interaction between Humic Acid and Ciprofloxacin [J]. Acta Chimica Sinica, 2021, 79(12): 1494-1501.
[11]	Ma Minghao, Xu Ming, Liu Sijin. Surface Chemical Modifications of Graphene Oxide and Interaction Mechanisms at the Nano-Bio Interface [J]. Acta Chimica Sinica, 2020, 78(9): 877-887.
[12]	Sun Qi, Xiao Feng-Shou. Exploration of Porous Organic Polymers as a Platform for Biomimetic Catalysis [J]. Acta Chimica Sinica, 2020, 78(9): 827-832.
[13]	Li Haimei, Luo Huajian, Xiao Qi, Yang Liyun, Huang Shan, Liu Yi. Investigations of Interactions and Mechanisms of Chiral Graphene Quantum Dots with DNA [J]. Acta Chimica Sinica, 2020, 78(6): 577-586.
[14]	Qi Meiwei, Liu Yong, Zhou Yongfeng. A Supramolecular Janus Hyperbranched Polymer and Its Electrochemically Responsive Self-Assembly Behavior [J]. Acta Chimica Sinica, 2020, 78(6): 528-533.
[15]	Liao Gang, Wu Yong-Jie, Shi Bing-Feng. Noncovalent Interaction in Transition Metal-Catalyzed Selective C-H Activation [J]. Acta Chimica Sinica, 2020, 78(4): 289-298.

糖芯片技术研究琼胶寡糖与凝集素RCA₁₂₀相互作用

Interaction of Agarose-based Oligosaccharides with Agglutinin RCA₁₂₀ Using Glycochip Technology

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

糖芯片技术研究琼胶寡糖与凝集素RCA120相互作用