Acta Chimica Sinica ›› 2004, Vol. 62 ›› Issue (18): 1829-1834. Previous Articles     Next Articles

巨大芽孢杆菌D01吸附金(Au3+)的谱学表征

林种玉1, 吴剑鸣1, 傅博强1, 薛茹1, 周剑章1, 郑泉兴1, 刘月英2, 傅锦坤1   

  1. 1. 厦门大学化学系, 固体表面物理化学国家重点实验室, 厦门, 361005;
    2. 厦门大学化学系, 生物系, 厦门, 361005
  • 投稿日期:2004-03-25 修回日期:2004-06-24 发布日期:2014-02-17
  • 通讯作者: 林种玉,E-mail:stzylin@xnu.edu.cn;Fax:0592-2183047. E-mail:stzylin@xnu.edu.cn

Spectroscopic Characterization on Interaction of Gold (Au3+) Biosorption by Bacillus megaterium DO1

LIN Zhong-Yu1, WU Jian-Ming1, FU Bo-Qiang1, XUE Rua1, ZHOU Jian-Zhang1, ZHENG Quan-Xing1, LIU Yue-Ying2, FU Jin-Kun1   

  1. 1. Department of Chemistry, State Key Laboratory of Physical Chemistry of Solid Surfaces, Xiamen University, Xiamen 361005;
    2. Department of Biology, Xiamen University, Xiamen 361005
  • Received:2004-03-25 Revised:2004-06-24 Published:2014-02-17

Biosorptive interaction of gold with resting cell of Bacillus megatherium D01 biomass has been characterized by spectroscopic techniques. The effects of pH, time and temperature on the correlation parameters of chemical kinetics and thermodynamics of the binding reaction has been investigated through the determination of the gold ion binding to the biomass using atomic absorption spectrophotometry (AAS). The analysis for sulfur content in dry powder of the D01 biomass by energy dispersive X-ray (EDX) shows that cysteine and methionine being capable of reducing Au3+ to Au0 is very small, whereas the glucose content in hydrolysates of the biomass analyzed by ultraviolet-visible spectrophotometry (UV-vis) indicates that the amount of the reducing sugars in the biomass is much larger than 3.33%. The Fourier transform infrared (FTIR) spectroscopy on blank and gold-loaded biomass demonstrates that the active groups such as the hydroxyl group of saccharides and the ionized carboxyl group of amino acid residues on the cell wall seem to be the sites for Au3+ binding, and the free aldehyde group of the hemiacetalic hydroxyl group from reducing sugars, i.e. the hydrolysates of the polysaccharides, serving as the electron donor, in situ reduces the Au3+ to Au0. X-ray powder diffractometry (XRD) and FTIR spectroscopic characterizations of the interaction of Au3+ with glucose confirm that the reduction of Au3+ to Au0can directly occur in the aldehyde group of the reducing sugars.

Key words: biosorption, Bacillus megatherium, gold, bioreduction, FTIR