Study on Fourier Transform Surface-Enhanced Raman Scattering of Immunoassay Using Mercapto Molecules as Reporter-labels

Acta Chimica Sinica ›› 2004, Vol. 62 ›› Issue (20): 2118-2122. Previous Articles Next Articles

巯基苯类为标记分子免疫检测的FT-SERS光谱研究

李淑瑾, 顾仁敖

苏州大学化学系, 苏州, 215006

投稿日期:2004-03-03 修回日期:2004-05-25 发布日期:2014-02-17
通讯作者: 顾仁敖,E-mail:ragu@suda.edu.cn E-mail:ragu@suda.edu.cn
基金资助:
国家自然科学基金(Nos.20273045,20373046)和厦门大学固体表面物理化学国家重点实验室开放基金资助项目.

Study on Fourier Transform Surface-Enhanced Raman Scattering of Immunoassay Using Mercapto Molecules as Reporter-labels

LI Shu-Jin, GU Ren-Ao

Department of Chemistry, Suzhou University, Suzhou 215006

Received:2004-03-03 Revised:2004-05-25 Published:2014-02-17

Abstract

Gold colloids were labeled with Raman reporter mercapto molecules such as 4-mercaptobenzoic acid and thiophenol to form Fourier transform (FT) surface-enhanced Raman scattering (SERS) reporter-labeled gold colloids. Firstly, antibody goat anti-rat was adsorbed onto the reporter-labeled gold colloids to form reporter-labeled immunogold colloids in appropriate condition. Secondly, the glass slide which was assembled gold nanoparticles was coated with antibody and selectively adsorbed antigen rat IgG to form antibody-antigen couple, and the SERS reporter-labeled immunogold colloids were modified onto the couple in a glass slide with gold nanoparticles adsorbing "antibody/antigen/reporter-labeled immunogold colloids" to form sandwich-type complex through the specific adsorption between antibody and antigen. The selective interaction between antibody and antigen can be read out by the labeling molecules.

Key words: 4-mercaptobenzoic acid, thiophenol, surface enhanced Raman scattering, gold nanoparticle, immunoassay

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LI Shu-Jin, GU Ren-Ao. Study on Fourier Transform Surface-Enhanced Raman Scattering of Immunoassay Using Mercapto Molecules as Reporter-labels[J]. Acta Chimica Sinica, 2004, 62(20): 2118-2122.

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巯基苯类为标记分子免疫检测的FT-SERS光谱研究

Study on Fourier Transform Surface-Enhanced Raman Scattering of Immunoassay Using Mercapto Molecules as Reporter-labels

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