A Novel Near-infrared Responsive Lanthanide Upconversion Nanoplatform for Drug Delivery Based on Photocleavage of Cypate※

doi:10.6023/A22010001

Acta Chimica Sinica ›› 2022, Vol. 80 ›› Issue (4): 423-427.DOI: 10.6023/A22010001 Previous Articles Next Articles

Special Issue: 中国科学院青年创新促进会合辑

Communication

基于cypate光裂解的新型近红外光响应稀土上转换纳米载药系统^※

刘若湄^a^,^b, 冯艳辉^a^,^b, 李卓^b, 卢珊^a^,^b^,^*(), 关天用^b, 李幸俊^b, 刘䶮^b, 陈卓^b, 陈学元^a^,^b^,^*()

a 福州大学化学学院福州 350108
b 中国科学院福建物质结构研究所中国科学院功能纳米结构设计与组装重点实验室福州 350002

投稿日期:2022-01-01 发布日期:2022-04-28
通讯作者: 卢珊, 陈学元
作者简介:
^※ 庆祝中国科学院青年创新促进会十年华诞.
基金资助:
国家自然科学基金(12174392); 国家自然科学基金(22175179); 国家自然科学基金(22135008); 福建省自然科学基金(2021I0040); 福建省自然科学基金(2021L3024); 福建省自然科学基金(2021Y0067)

A Novel Near-infrared Responsive Lanthanide Upconversion Nanoplatform for Drug Delivery Based on Photocleavage of Cypate^※

Ruomei Liu^a^,^b, Yanhui Feng^a^,^b, Zhuo Li^b, Shan Lu^a^,^b(), Tianyong Guan^b, Xingjun Li^b, Yan Liu^b, Zhuo Chen^b, Xueyuan Chen^a^,^b()

a College of Chemistry, Fuzhou University, Fuzhou 350108, China
b CAS Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, China

Received:2022-01-01 Published:2022-04-28
Contact: Shan Lu, Xueyuan Chen
About author:
^※ Dedicated to the 10th anniversary of the Youth Innovation Promotion Association, CAS.
Supported by:
National Natural Science Foundation of China(12174392); National Natural Science Foundation of China(22175179); National Natural Science Foundation of China(22135008); Natural Science Foundation of Fujian Province(2021I0040); Natural Science Foundation of Fujian Province(2021L3024); Natural Science Foundation of Fujian Province(2021Y0067)

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Abstract

Light-responsive drug delivery systems (DDS) exhibit the advantages of non-invasive, high controllability and spatio-temporal precision. However, DDS triggered by near-infrared (NIR) light are few and inefficient. In this work, we designed a novel NIR-responsive upconversion nanoplatform for drug delivery. In this nanoplatform, core-shell upconversion nanoparticle (UCNP) NaYF₄:Yb,Er@NaYF₄ was coated by mesoporous silica, and then successively coupled with NIR dye cypate, amantadine (AD) and β-cyclodextrin (β-CD) to block the pores and entrap the drugs. The cypate molecules with the feature of auto-sensitized photooxidation under 808 nm irradiation were, for the first time, employed as light-responsive moieties in DDS. The obtained nanoplatform was characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), N₂ adsorption/desorption, dynamic light scattering (DLS) and zeta potential analysis. Mechanism of photocleavage of cypate by singlet oxygen (¹O₂) was also investigated by electron spin resonance (ESR) measurement. The nanoplatform loaded with antibiotic ofloxacin (OFL) showed a low drug leakage (6.9%) in the dark condition and a rapid release (50.9%) upon 808 nm irradiation with a relatively low power density of 0.5 W•cm^-2 for 40 min. Moreover, on-demand release of OFL can be achieved by adjusting the irradiation time (0~40 min). In vitro antibacterial experiments showed that the nanoplatform had a much better antibacterial effect against Staphylococcus aureus after 808 nm irradiation as compared with the group without irradiation. These results further verified the excellent NIR-responsive performance for the designed nanoplatform. In addition, the nanoplatform exhibited strong and stable upconversion luminescence (UCL) under 980 nm excitation, which can be applied for DDS tracing and bioimaging. The cytocompatibility of the nanoplatform was evaluated by methyl thiazolyl tetrazolium (MTT) assay, showing that the nanoplatform had no cytotoxic effect on human embryonic liver cell line (LO2) and exhibited great potentials in versatile bioapplications. Our work may open up a new avenue for the exploration of multi-functional NIR-responsive DDS.

Key words: upconversion nanoparticles, near-infrared light-responsive, near-infrared dye, drug delivery, photocleavage

Cite this article

Ruomei Liu, Yanhui Feng, Zhuo Li, Shan Lu, Tianyong Guan, Xingjun Li, Yan Liu, Zhuo Chen, Xueyuan Chen. A Novel Near-infrared Responsive Lanthanide Upconversion Nanoplatform for Drug Delivery Based on Photocleavage of Cypate^※[J]. Acta Chimica Sinica, 2022, 80(4): 423-427.

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Fig. & Tab. 4

Figure 1. Illustration of the fabrication of near-infrared (NIR) light responsive upconversion nanoplatform for drug delivery

Figure 2. TEM images of (A) UCNP and (B) UCNP@MS. (C) XRD patterns of UCNP and UCNP@MS. (D) N2 adsorption/desorption isotherm and pore size distribution of UCNP@MS. (E) Zeta potentials of UCNP@MS, UCNP@MS-NH2, UCNP@MS-NH2-cypate. (F) Hydrodynamic size distributions of UCNP@MS, UCNP@MS-cypate-AD and OFL-UCNP@MS-cypate@β-CD. (G) UV-Vis-NIR absorption spectra of UCNP@MS, UCNP@MS-cypate-AD, OFL-UCNP@MS-cypate@β-CD and OFL. (H) FT-IR spectra of UCNP@MS, UCNP@MS-cypate-AD, and OFL-UCNP@MS-cypate@β-CD

Figure 3. (A) Variation of absorption spectra of OFL-UCNP@MS-cypate@β-CD under excitation at 808 nm. (B) Comparison of photocleavage efficiency of cypate in different nanoplatforms. (C) Mechanism of photocleavage of cypate by 1O2. (D) ESR spectrum of OFL-UCNP@MS-cypate@β-CD after 808 nm laser irradiation

Figure 4. (A) Absorption spectra of OFL released from nanoplatform with time under 808 nm irradiation. (B) Release profiles of OFL from nanoplatform with different irradiation time of 808 nm. (C) Upconverson luminescence (UCL) spectra and corresponding UCL photographs of nanoplatform excited by 980 nm before and after drug release. (D) Antibacterial effect on Staphylococcus aureus (SA) with different treatments. (E) CLSM images of SA incubated with nanoplatform: (i) bright-field image, (ii) green UCL image excited by 980 nm, and (iii) the overlay image

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基于cypate光裂解的新型近红外光响应稀土上转换纳米载药系统^※

A Novel Near-infrared Responsive Lanthanide Upconversion Nanoplatform for Drug Delivery Based on Photocleavage of Cypate^※

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基于cypate光裂解的新型近红外光响应稀土上转换纳米载药系统※

A Novel Near-infrared Responsive Lanthanide Upconversion Nanoplatform for Drug Delivery Based on Photocleavage of Cypate※

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Supporting Info.

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Abstract

Cite this article

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References 36

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基于cypate光裂解的新型近红外光响应稀土上转换纳米载药系统^※

A Novel Near-infrared Responsive Lanthanide Upconversion Nanoplatform for Drug Delivery Based on Photocleavage of Cypate^※