多肽酰肼法合成翻译后修饰物蛋白NEDD8

doi:10.6023/cjoc201605013

有机化学 ›› 2016, Vol. 36 ›› Issue (11): 2763-2768.DOI: 10.6023/cjoc201605013 上一篇下一篇

研究简报

多肽酰肼法合成翻译后修饰物蛋白NEDD8

管超建, 王涛, 王君, 李宜明

合肥工业大学生物与医学工程学院合肥 230009

收稿日期:2016-05-10 修回日期:2016-06-08 发布日期:2016-07-07
通讯作者: 王君, 李宜明 E-mail:wangjun-08@126.com;lym2007@mail.ustc.edu.cn
基金资助:
国家自然科学基金（Nos.21372058，21572043）资助项目.

Synthesis of Post-translational Modifier Protein NEDD8 via Ligation of Peptide Hydrazides

Guan Chaojian, Wang Tao, Wang Jun, Li Yiming

Department of Pharmacy, School of Biological and Medical Engineering, Hefei University of Technology, Hefei 230009

Received:2016-05-10 Revised:2016-06-08 Published:2016-07-07
Supported by:
Project supported by the National Natural Science Foundation of China (Nos.21372058,21572043).

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1. 多肽酰肼法合成翻译后修饰物蛋白NEDD8.PDF(856KB)

摘要/Abstract

NEDD8（神经前体细胞表达发育下调蛋白8）是真核生物体内一种重要的类泛素化蛋白，参与调控细胞内一系列重要的生命过程.目前获得NEDD8的主要方法是生物重组表达，该策略的缺点在于表达量不高且需要除去外加纯化标签的步骤.本研究结合高温辅助固相合成技术与基于多肽酰肼连接的一锅连接-脱硫策略，较为高效地获得了均一性的NEDD8蛋白，为进一步研究NEDD8修饰蛋白的结构与功能提供了基础.

关键词: NEDD8, 高温辅助固相合成, 多肽酰肼连接, 一锅连接脱硫

As an important ubiquitin-like modifier protein in eukaryotic organisms, NEDD8 (neural precursor cell expressed developmentally down-regulated 8) is involved in regulating a series of important life processes in cells. Nowadays, the major method for obtaining NEDD8 is recombinant protein expression. However, the yield is relatively low and the recombined tag for purification needs to be removed in an extra step. In present work, NEDD8 protein was first synthesized in homogeneity by using high temperature assisted solid-phase peptide synthesis (SPPS) combining with the one-pot ligation-desulfurization strategy. This method lays the foundation for the study of NEDD8 modified proteins in future.

Key words: NEDD8, high temperature assisted SPPS, ligation of peptide hydrazide, one-pot ligation-desulfurization

引用此文

管超建, 王涛, 王君, 李宜明. 多肽酰肼法合成翻译后修饰物蛋白NEDD8[J]. 有机化学, 2016, 36(11): 2763-2768.

Guan Chaojian, Wang Tao, Wang Jun, Li Yiming. Synthesis of Post-translational Modifier Protein NEDD8 via Ligation of Peptide Hydrazides[J]. Chin. J. Org. Chem., 2016, 36(11): 2763-2768.

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多肽酰肼法合成翻译后修饰物蛋白NEDD8

Synthesis of Post-translational Modifier Protein NEDD8 via Ligation of Peptide Hydrazides

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