有机化学 ›› 1983, Vol. 3 ›› Issue (2): 101-106. 上一篇    下一篇

研究论文

胆汁酸的薄层层析--数理统计法用于层析溶剂系统的选择

郑崇直;罗国芳;管重庆;王大琛   

  1. 中国科学院上海有机化学研究所
  • 发布日期:1983-04-25

Thin layer chromatography of bile acids -- selection of solvent system by statistics

ZHENG CHONGZHI;LUO GUOFANG;GUAN CHONGQING;WANG DACHEN   

  • Published:1983-04-25

关键词: 薄层色谱, 应用, 正交设计, 数理统计, 胆酸

The application of statistics in the selection of developing solvent in TLC is reported. Chloroform, ethanol, ethylacetate, acetic acid and water were used as solvents, and experiments were arranged in accordance with the orthogonal design method (L16(4**5)) The Rf values and the concentrations of spots of cholesterol, cholic acid, deoxycholic acid, glycocholic acid, glycodeoxycholic acid, taurocholic acid, taurodeoxycholic acid and lecithin have been analysed by the method of variance, and two effective solvent systems for uni-dimension TLC were obtained, by which the spots were found to be distributed uniformly. The results obtaind were better than that by methods reported in the literature.

Key words: THIN-LAYER CHROMATORGAPHY, APPLICATION, ORTHOGONAL DESIGN, MATHEMATICAL STATISTICS, BILE ACID

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