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有机化学
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有机化学 ›› 1997, Vol. 17 ›› Issue (6): 498-506. 上一篇    下一篇

研究论文

基质辅助激光解吸离子化质谱及其在生物大分子分析中的应用

吴忆南;陈耀祖;潘远江;沈飙   

  1. 浙江大学化学系;兰州大学;应用有机国家重点实验室
  • 发布日期:1997-12-25

Matrix-assisted laser desorption ionization mass spectrometry and its applications in biomacromolecular analysis

WU YINAN;CHEN YAOZU;PAN YUANJIANG;SHEN BIAO   

  • Published:1997-12-25

被引次数

17

近年来质谱离子化技术方面有两项重要成果：基质辅助激光解吸离子化(matrix-assisted laser desorption ionization，MALDI)和电喷雾离子化(electrospray ionization，ESI)。MALDI和ESI的应用使质谱在生物大分子研究方面取得重大突破。本文仅就MALDI的原理、特点、样品准备方法、基质的选择、仪器条件及其在生物大分子应用方面的最新进展进行简要的综述。

关键词: 离子化, 飞行时间质谱法, 质谱法, 国家教委高等学校博士学科点专项科研基金, 质谱研究, 解吸, 激光, 生物大分子

In last few years, two important achievements of ionization techniques in mass spectrometry have been developed: matrix- assisted laser desorption ionization (MALDI) and electrospray ionization (ESI). By these innovative techniques, mass spectrometry becomes one of the most important experimental tools for the analysis of biomacromoleculars. In this article, we briefly review the principles, characteristics, sample preparation, matrix selection, instrumentation of MALDI and its current progress in biomacromolecular analysis.

Key words: TIME-OFF FLIGHT MASS SPECTROMETRY, DESORPTION, MASS SPECTROGRAPHY, IONISE (=IONIZE), LASER, BIOMACROMOLECULE, MASS SPECTROGRAPHY

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