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2013 , Vol. 33 >Issue 10: 2210 - 2215

DOI: https://doi.org/10.6023/cjoc201305013

研究论文

可逆识别Hg2+的罗丹明基荧光增强型探针及在活细胞成像中的应用

  • 陈稼轩 ,
  • 田怡 ,
  • 向清祥 ,
  • 熊俊如 ,
  • 张丽群 ,
  • 李艳
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  • 乐山师范学院化学学院 乐山 614000

收稿日期: 2013-05-10

  修回日期: 2013-05-31

  网络出版日期: 2013-06-09

基金资助

四川省科技厅应用基础(No. 2009JY0078);四川省教育厅重点(No. 11ZA154)和乐山市科技局重点(No. 2011SZD107)资助项目

收起

A Reversible Fluorescence Enhancement Probe for Hg2+ Based on Rhodamine and Its Application in Live Cell Imaging

  • Chen Jiaxuan ,
  • Tian Yi ,
  • Xiang Qingxiang ,
  • Xiong Junru ,
  • Zhang Liqun ,
  • Li Yan
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  • Department of Chemistry, Leshan Normal University, Leshan 614000

Received date: 2013-05-10

  Revised date: 2013-05-31

  Online published: 2013-06-09

Supported by

Project supported by the Science and Technology Department (No. 2009JY0078), the Education Department of Sichuan Province (No. 11ZA154), and the Science and Technology Bureau of Leshan City (No. 2011SZD107).

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摘要

基于罗丹明类荧光团, 合成了一个新型的Hg2+荧光探针RS. 通过紫外光谱法和荧光光谱法研究了探针RS在EtOH-HEPES缓冲液(V:V=1:1, pH=7.3)中对Hg2+的识别特性. 结果表明: 探针RS可快速、可逆识别Hg2+. 随着Hg2+的滴加, 探针RS的内酰胺环结构发生开环, 并形成1:1 Hg2+配合物. 在L929活细胞中的荧光显微成像表明RS可应用于生物体的检测.

关键词： 罗丹明B; 荧光探针; 可逆性; Hg2+离子; 活细胞

本文引用格式

陈稼轩 , 田怡 , 向清祥 , 熊俊如 , 张丽群 , 李艳 . 可逆识别Hg2+的罗丹明基荧光增强型探针及在活细胞成像中的应用[J]. 有机化学, 2013 , 33(10) : 2210 -2215 . DOI: 10.6023/cjoc201305013

Abstract

A novel of Hg2+ fluorescence probe (RS) with rhodamine chromophore was synthesized, and its structure was characterized by 1H NMR, MS spectra and elemental analysis. The recognition properties of the probe RS with Hg2+ ions had been investigated in EtOH-HEPES buffer solution (V:V=1:1, pH=7.3) by the ultraviolet spectrophotometry and the fluorescence spectrophotometry. The results showed that the recognition is instantaneous and reversible. Upon the addition of Hg2+, the spirolactam ring of probe RS was opened and 1:1 ligand-mercury complex was formed. The fluorescence imaging experiments of Hg2+ in L929 living cells demonstrated that the probe RS could be used in biological systems.

Key words： rhodamine B; fluorescent probe; reversibility; Hg2+ ions; living cells

参考文献

[1] Renzoni, A.; Zino, F., Franchi, E. Environ. Res. 1998, 77(2), 68.
[2] Boening, D. W. Chemosphere 2000, 40(12), 1335.
[3] Harada, M.; Minamata, D. Crit. Rev. Toxicol. 1995, 25(1), 1.
[4] Nendza, M.; Herbst, T.; Kussatz, C.; Gies, A. Chemosphere 1997, 35, 1875.
[5] Clarkson, T. W. Am. J. Clin. Nutr. 1995, 61, 682.
[6] Li, N.; Liu, M.; Yin, W.-T.; Yang, Z.; Li, J.-L.; Shi, Z. Chin. J. Org. Chem. 2011, 31, 39 (in Chinese).(李娜, 刘美玲, 尹文婷, 杨征, 李剑利, 史真, 有机化学, 2011, 31, 39.)
[7] Chen, J.-X.; Tian, Y.; Xiang, Q.-X.; Zhang, L.-Q.; Xiong, J.-R. Chin. J. Org. Chem. 2012, 32, 1930 (in Chinese). (陈稼轩, 田怡, 向清祥, 张丽群, 熊俊如, 有机化学, 2012, 32, 1930.)
[8] Mariana, B.; Carlos, A. M. A.; José, M. G. M. Chem. Soc. Rev. 2009, 38, 2410.
[9] Huang, W.-J.; Wu, W.-H.; Liang, J.-X. Acta Chim. Sinica 2012, 70, 873 (in Chinese).(黄文君, 吴文辉, 梁嘉香, 化学学报, 2012, 70, 873.)
[10] Zhang, Q.; Xu, J. Acta Chim. Sinica 2011, 69, 2287 (in Chinese).
(张强, 许娟, 化学学报, 2011, 69, 2287.)
[11] Lv, S.-Y.; Ye, T.; Jiang, X.; Wang, J.-J.; Wei, G.-F.; Lv, J.-Q. Acta Chim. Sinica 2011, 69, 831 (in Chinese).
(吕诗言, 叶泰, 姜欣, 王晶晶, 魏国芬, 吕鉴泉, 化学学报, 2011, 69, 831.)
[12] Yang, Y. K.; Yook, K. J.; Tae, J. J. Am. Chem. Soc. 2005, 127, 16760.
[13] Wang, F.; Nam, S. W.; Guo, Z. Q.; Park, S. S.; Yoon, J. Y. Sens. Actuators B 2012, 161, 948.
[14] Gong, Y. J.; Zhang, X. B.; Chen, Z.; Yuan, Y.; Jin, Z.; Mei, L.; Zhang, J.; Tan, W. H.; Shen, G. L.; Yu, R. Q. Analyst 2012, 137, 932.
[15] Liu, W. M.; Chen, J. H.; Xu, L. W.; Wu, J. S.; Xu, H. T.; Zhang, H. Y.; Wang, P. F. Spectrochim. Acta, A 2012, 85, 38.
[16] Wang, H. G.; Li, Y. P.; Xu, S. F.; Li, Y. C.; Zhou, C.; Fei, X. L.; Sun, L.; Zhang, C. Q.; Li, Y. X.; Yang, Q. B.; Xu, X. Y. Org. Biomol. Chem. 2011, 9, 2850.
[17] Kim, H. N.; Nam, S. W.; Swamy, K. M. K.; Jin, Y.; Chen, X. Q.; Kim, Y. M.; Kim, S. J.; Park, S. S.; Yoon, J. Y. Analyst 2011, 136, 1339.
[18] Zhan, X. Q.; Qian, Z. H.; Zheng, H.; Su, B. Y.; Lan, Z.; Xu, J. G. Chem. Commun. 2008, 16, 1859.
[19] Zheng, H.; Qian, Z. H.; Xu, L.; Yuan, F. F.; Lan, L. D.; Xu, J. G. Org. Lett. 2006, 8, 859.
[20] Tang, L. J.; Li, F. F.; Liu, M. H.; Nandhakumar, R. Spectrochim. Acta A 2011, 78, 1168.
[21] Yang, Z.; She, M. Y.; Yin, B.; Cui, J. H.; Zhang, Y. Z.; Sun, W.; Li, J. L.; Shi, Z. J. Org. Chem. 2012, 77(2), 1143.
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