红树尖瓣海莲内生真菌PhomopsislongicollaHL-2232次级代谢产物研究
收稿日期: 2015-05-27
修回日期: 2015-06-15
网络出版日期: 2015-06-24
基金资助
国家自然科学基金(Nos. 21462015, 21362009, 31360069)、海南省自然科学基金(Nos. 20152037, 20152034)及海南师范大学科研启动基金(No. QN1434)资助项目.
Secondary Metabolites of a Bruguiera sexangula var. Rhynchopetala-Derived Fungus Phomopsis longicolla HL-2232
Received date: 2015-05-27
Revised date: 2015-06-15
Online published: 2015-06-24
Supported by
Project supported by the National Natural Science Foundation of China (Nos. 21462015,21362009, 31360069), the Natural Science Foundation of Hainan Province (Nos. 20152037, 20152034), and the Youth Foundation of Hainan Normal University (No. QN1434).
通过活性追踪的方法, 从一株来源于药用红树尖瓣海莲的内生真菌Phomopsis longicolla HL-2232中分离鉴定了5个生物碱类化合物、1个色原酮类化合物以及4个甾醇类化合物, 分别鉴定为: 6-氨基嘌呤-9-羧酸甲酯(1), 腺嘌呤核苷(2), 尿嘧啶核苷(3), N,N'-二苯基尿素(4), (2S,2'R,3R,4E,8E,3'E)-2-(2'-羟基-3'-十八碳烯酰胺)-9-甲基-4,8-十八碳二烯-1,3-二醇(5), 2-(2'S-羟丙基)-5-甲基-7-羟基对氧萘酮(6), fortisterol (7), (22E)-5α,8α-表二氧麦角甾-6,22-二烯-3β-醇(8), 啤酒甾醇(9), β-谷甾醇亚油酸酯(10). 其中化合物1为新化合物, 化合物5为新天然产物, 其碳谱数据至今未曾报道. 细胞毒活性表明化合物1～3对肿瘤细胞A549, B16F10, HL-60, MCF-7具有不同程度的抑制活性. 其中新化合物1对乳腺癌细胞(MCF-7)的IC50值为14.9 μmol·L-1、化合物3对肺癌细胞(A549)的IC50值为8.6 μmol·L-1, 两者活性强于阳性对照药顺铂.
关键词： 尖瓣海莲; Phomopsis longicolla; 次级代谢产物; 生物碱; 细胞毒活性
宋鑫明 , 周学明 , 李小宝 , 郑彩娟 , 黄国雷 , 余章昕 , 宋小平 , 陈光英 . 红树尖瓣海莲内生真菌PhomopsislongicollaHL-2232次级代谢产物研究[J]. 有机化学, 2015 , 35(10) : 2102 -2107 . DOI: 10.6023/cjoc201505044
Under the guidance of bioassay, five alkaloids, one chromone and four steroids were isolated from the Phomopsis longicolla HL-2232, a fungus isolated from a mangrove Bruguiera sexangula var. Rhynchopetala. Their structures were identified as 6-aminopurine-9-carboxylic acid methyl ester (1), adenine riboside (2), uridine (3), N,N'-diphenyl urea (4), (2S,2'R,3R,4E,8E,3'E)-2-(2'-hydroxy-3'-octadecenoylamino)-9-methyl-4,8-octadecadiene-l,3-diol (5), 2-(2'S-hydroxypropyl)-5-methyl-7-hydroxychromone (6), fortisterol (7), (22E)-5α,8α-epidioxyergosta-6,22-dien-3β-ol (8), cerevisterol (9) and β-sitosteryl linoleate (10). Among them, compound 1 was a new compound. Compound 5 was a new natural product, and its 13C NMR spectroscopic data has not reported until now. Compounds 1～3 showed inhibitory activities against B16F10, A549, HL-60 and MCF-7. Compounds 1 and 3 exhibited the higher inhibitory activities against MCF-7 and A549 than positive control cisplatin with IC50 values of 14.9 and 8.6 μmol·L-1, respectively.
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