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2020 , Vol. 40 >Issue 4: 1038 - 1042

DOI: https://doi.org/10.6023/cjoc201908033

研究简报

疣孢菌NS0172产生的邻二烷基取代芳香酸

  • 张坤 ,
  • 谢向前 ,
  • 史海霞 ,
  • 沈月毛 ,
  • 王浩鑫
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  • a 山东大学微生物技术研究院 微生物技术国家重点实验室 青岛 266237;
    b 山东大学药学院 天然产物化学生物学教育部重点实验室 济南 250012

收稿日期: 2019-08-26

  修回日期: 2019-11-08

  网络出版日期: 2020-05-06

基金资助

国家自然科学基金（Nos.31570039，81530091）和教育部长江学者与创新团队发展计划（No.IRT_17R68）资助项目.

收起

ortho-Dialkyl-Substituted Aromatic Acids from Verrucosispora sp. NS0172

  • Zhang Kun ,
  • Xie Xiangqian ,
  • Shi Haixia ,
  • Shen Yuemao ,
  • Wang Haoxin
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  • a State Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, Qingdao 266237;
    b Key Laboratory of Chemical Biology(Ministry of Education), School of Pharmaceutical Sciences, Shandong University, Jinan 250012

Received date: 2019-08-26

  Revised date: 2019-11-08

  Online published: 2020-05-06

Supported by

Project supported by the National Natural Science Foundation of China (Nos. 31570039, 81530091), and the Program for Changjiang Scholars and Innovative Research Team in University (No. IRT_17R68).

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摘要

从疣孢菌NS0172的发酵产物中分离得到2个新的邻二烷基取代芳香酸类化合物1和2.通过一维和二维NMR数据解析及高分辨质谱分析确定了化合物1和2的化学结构.利用噻唑蓝（MTT）比色法测定了化合物1和2的细胞毒活性，结果显示化合物1对人肝癌细胞SMMC-7721具有一定的细胞毒性（IC50 7.74 μmol·L-1）.利用滤纸片法测定了化合物1和2的抗细菌和抗真菌活性，结果显示两者在40 μg/disc条件下均无抗菌活性.这是首次报道疣孢菌代谢产生邻二烷基取代芳香酸类化合物，为后续开展相关生物合成机制研究奠定了基础.

关键词： 疣孢菌; 放线菌; 天然产物; 邻二烷基取代芳香酸

本文引用格式

张坤 , 谢向前 , 史海霞 , 沈月毛 , 王浩鑫 . 疣孢菌NS0172产生的邻二烷基取代芳香酸[J]. 有机化学, 2020 , 40(4) : 1038 -1042 . DOI: 10.6023/cjoc201908033

Abstract

Two new ortho-dialkyl-substituted aromatic acids 1 and 2 were isolated from Verrucosispora sp. NS0172. The chemical structures of 1 and 2 were determined by spectroscopic methods including 1D- and 2D-NMR and HR-ESIMS experiments. The cytotoxicity of compounds 1 and 2 was evaluated by methyl thiazolyl tetrazolium (MTT) assay, and compound 1 showed potent antiproliferative activity against human hepatocellular carcinoma SMMC-7721 (IC50 7.74 μmol·L-1). Compounds 1 and 2 were tested for the antimicrobial and antifungal activities by filter paper disc diffusion assay, and both of them showed no evident activities at a dose of 40 μg/disc. This study is the first report of discovering of ortho-dialkyl-substi-tuted aromatic acids from Verrucosispora, which sets the foundation for future biosynthetic study of this class of natural products in Verrucosispora.

Key words： verrucosispora; actinomycetes; natural product; ortho-dialkyl-substituted aromatic acids

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