江西嗜酸链霉菌去铁胺E及金属络合物的分离鉴定与生物活性研究
收稿日期: 2024-01-30
修回日期: 2024-03-11
网络出版日期: 2024-04-10
基金资助
科技部重大专项(2022YFC2303100); 国家自然科学基金(22193070); 国家自然科学基金(32030002)
Study on Isolation, Identification and Bioactivity of Desferrioxamine E and Its Metal-Complexes from Streptacidiphilus jiangxiensis
Received date: 2024-01-30
Revised date: 2024-03-11
Online published: 2024-04-10
Supported by
National Key Research and Development Program of China(2022YFC2303100); National Natural Science Foundation of China(22193070); National Natural Science Foundation of China(32030002)
嗜酸链霉菌属细菌的研究主要集中在新物种的分离和鉴定上, 而其次级代谢产物的研究则相对较少. 从江西嗜酸链霉菌的发酵液中分离得到一个新的铝配合物铝草铵(alumioxamine, 3)和两个已知化合物去铁胺E (desferrioxamine E, 1)和铁草铵E (ferrioxamine E, 2), 并通过MS、IR、NMR以及单晶X射线衍射技术对其结构进行鉴定. 从结构上来看, 化合物3以铝为中心, 与配体的六个O原子相连, 形成六齿配合物. 此外, 体外细胞毒活性实验表明, 在100 μmol/L浓度时化合物1对4种人肿瘤细胞(Hela、PANC1、A375和MHCC-97H)均具有一定的抑制活性, 细胞存活率为4.8%~66.4%.
李金达 , 程伯涛 , 黄积武 , 刘文 . 江西嗜酸链霉菌去铁胺E及金属络合物的分离鉴定与生物活性研究[J]. 有机化学, 2024 , 44(7) : 2377 -2380 . DOI: 10.6023/cjoc202401038
Research on the genus Streptacidiphilus was mainly focused on the isolation and identification of new species, with relatively less emphasis on the study of secondary metabolites. A new aluminium-complex alumioxamine (3), and two known compounds desferrioxamine E (1) and ferrioxamine E (2) were isolated from the fermentation broth of Streptacidiphilus jiangxiensis CGMCC 4.1857. Their structures were elucidated by MS, IR, NMR and single crystal X-ray diffraction technologies. Structurally, compound 3 features aluminum(III) as the central atom, which is bonded to six oxygen atoms of the ligand, forming a hexadentate complex. Furthermore, in vitro cytotoxicity assays showed that compound 1 exhibited potential inhibitory activity against four human tumor cell lines (Hela, PANC1, A375 and MHCC-97H) at a concentration of 100 μmol/L with cell viability ranging from 4.8% to 66.4%.
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