运用DeepSAT预测和活性引导的方法对一株分离自青海可可西里的链霉菌(Streptomyces sp. 444)抗真菌活性成分进行研究,从中发现了两个新的抗霉素类化合物,去甲酰抗霉素A10a (1)、抗霉素A1c (2)和两个已知的抗霉素类化合物,抗霉素A3a (3)、抗霉素A8a (4)。通过高分辨质谱(HR-ESI-MS)、核磁共振技术(NMR)和NMR计算确定了新化合物的结构。微量肉汤稀释法测试表明4个化合物均表现出抗真菌活性,其中化合物2最强,对白色念珠菌Candida albicans ATCC 10231和新型隐球菌Cryptococcus neoformans H99的最低抑菌浓度MIC值分别为4和8 μg/mL。
安俊榴
,
徐吉祥
,
庞昆
,
杨信怡
,
孙承航
,
袁丽杰
. 可可西里来源链霉菌444的抗真菌活性成分研究[J]. 有机化学, 0
: 202604020
-202604020
.
DOI: 10.6023/cjoc202604020
Activity-guided and DeepSAT analyses on the antifungal constituents of Streptomyces sp. 444, isolated from the Hoh Xil region, led to the discovery of two new antimycin analogs, deformyl-antimycin A10a (1) and antimycin A1c (2), along with two known analogs, antimycin A3a (3) and antimycin A8a (4). The structures of these antimycin analogs were elucidated by high-resolution electrospray ionization mass spectroscopy (HR-ESI-MS), nuclear magnetic resonance (NMR), and NMR calculations. Bioassay indicated that compounds 1-4 exhibited antifungal activity. The MIC values of compound 2 against Candida albicans ATCC 10231 and Cryptococcus neoformans H99, determined by the broth microdilution method, were 4 and 8 μg/mL, respectively.
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