Study on the Secondary Metabolites and Antibacterial Activity of a Fungus Aspergillus sp. L06 from the Roots of Stelleropsis tianschanica (Pobed)

doi:10.6023/cjoc202511024

Chinese Journal of Organic Chemistry ›› 2026, Vol. 46 ›› Issue (6): 2482-2486.DOI: 10.6023/cjoc202511024 Previous Articles Next Articles

天山假狼毒根部来源曲霉Aspergillus sp. L06的次级代谢产物及抗菌活性研究

董莉萍^a^,^†, 杨金花^a^,^†, 方容^a, 张兴^a, 朱小强^a, 毕晓宾^b^,^*(), 雷亮^a^,^*()

^a 武汉市第四医院武汉 430033
^b 华中科技大学药学院武汉 430030

收稿日期:2026-02-03 修回日期:2026-03-02 发布日期:2026-04-02
作者简介:
^† 共同第一作者.
基金资助:
武汉市卫生健康委员会科研项目(WZ22Q21); 武汉市卫生健康委员会科研项目(WZ24B14); 国家自然科学基金(32000282); 国家自然科学基金(823041523)

Study on the Secondary Metabolites and Antibacterial Activity of a Fungus Aspergillus sp. L06 from the Roots of Stelleropsis tianschanica (Pobed)

Liping Dong^a, Jinhua Yang^a, Rong Fang^a, Xing Zhang^a, Xiaoqiang Zhu^a, Xiaobin Bi^b^,^*(), Liang Lei^a^,^*()

^a Wuhan Fourth Hospital, Wuhan 430033
^b School of Pharmacy, Huazhong University of Science and Technology, Wuhan 430030

Received:2026-02-03 Revised:2026-03-02 Published:2026-04-02
Contact: * E-mail: leiliang@hust.edu.cn;bxbin@hust.edu.cn
About author:
^† The authors contributed equally to this work.
Supported by:
Wuhan Municipal Health Commission(WZ22Q21); Wuhan Municipal Health Commission(WZ24B14); National Natural Science Foundation of China(32000282); National Natural Science Foundation of China(823041523)

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Abstract

This study aims to find monomer compounds with antimicrobial activity from the secondary metabolites of a soil-derived fungus Aspergillus sp. L06 isolated from the roots of Stelleropsis tianschanica (Pobed) in Xinjiang pastoral areas. Four compounds were obtained from the ethyl acetate layer of the fermentation of Aspergillus sp. L06 by silica gels, Sephadex LH-20, octadecylsilyl (ODS) column chromatography, and HPLC methods. Their structures were identified as aspever- chromone A (1), sterigmatocystin (2), nidurufin (3), and versicolorin B (4) by NMR, MS and other spectroscopic techniques. Among them, compound 1 is a new compound. The results of in vitro antibacterial activity indicated that compound 1 exhibited a strong inhibitory effect on multidrug-resistant Pseudomonas aeruginosa, with a minimum inhibitory concentration (MIC) of 4.0 μg/mL, which was superior to the positive control gentamicin (8.0 μg/mL).

Key words: Stelleropsis tianschanica (Pobed), Aspergillus sp. L06, secondary metabolites, structural identification, in vitro antibacterial activity

Cite this article

Liping Dong, Jinhua Yang, Rong Fang, Xing Zhang, Xiaoqiang Zhu, Xiaobin Bi, Liang Lei. Study on the Secondary Metabolites and Antibacterial Activity of a Fungus Aspergillus sp. L06 from the Roots of Stelleropsis tianschanica (Pobed)[J]. Chinese Journal of Organic Chemistry, 2026, 46(6): 2482-2486.

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References 21

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Position	δ_H (J in Hz)	δ_C
1
2		169.7
3	6.05 s	111.3
4		180.1
4a		109.2
5		162.1
6	6.64 d (2.3)	94.0
7		166.4
8	6.51 d (2.3)	97.2
8a		161.8
9	2.63 t (7.6)	34.3
10	1.75 p (7.5)	27.6
11	1.38~1.42 m	29.6
12	1.43~1.45 m	29.7
13	1.64 p (7.4)	25.8
14	2.32 t (7.4)	35.0
15		175.5
16
17	4.11 q (7.1)	61.4
18	1.24 t (7.1)	14.5
5-OCH₃	3.90 s	56.5
7-OCH₃	3.92 s	56.5

Position	δ_H (J in Hz)	δ_C
1
2		169.7
3	6.05 s	111.3
4		180.1
4a		109.2
5		162.1
6	6.64 d (2.3)	94.0
7		166.4
8	6.51 d (2.3)	97.2
8a		161.8
9	2.63 t (7.6)	34.3
10	1.75 p (7.5)	27.6
11	1.38~1.42 m	29.6
12	1.43~1.45 m	29.7
13	1.64 p (7.4)	25.8
14	2.32 t (7.4)	35.0
15		175.5
16
17	4.11 q (7.1)	61.4
18	1.24 t (7.1)	14.5
5-OCH₃	3.90 s	56.5
7-OCH₃	3.92 s	56.5

Compound	MRSE	MDR-AB	MDR-EC	MDR-PA
1	32.0	32.0	16.0	4.0
2	>64.0	>64.0	>64.0	>64.0
3	>64.0	>64.0	>64.0	>64.0
4	>64.0	>64.0	>64.0	>64.0
庆大霉素	2.0	8.0	4.0	8.0

Compound	MRSE	MDR-AB	MDR-EC	MDR-PA
1	32.0	32.0	16.0	4.0
2	>64.0	>64.0	>64.0	>64.0
3	>64.0	>64.0	>64.0	>64.0
4	>64.0	>64.0	>64.0	>64.0
庆大霉素	2.0	8.0	4.0	8.0

天山假狼毒根部来源曲霉Aspergillus sp. L06的次级代谢产物及抗菌活性研究

Study on the Secondary Metabolites and Antibacterial Activity of a Fungus Aspergillus sp. L06 from the Roots of Stelleropsis tianschanica (Pobed)

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