Enantiomers of 5 propargylic alcohols and 4 2, 3-allenic alcohols were studied for direct separaton by chiral gas chromatography (GC) using a permethylated β-cyclodextrin column (Rt-βDex^TM~cst, 30 m× 0.25 mm ID). Eight racemic alcohols (4 propargylic alcohols and 4 2, 3- allenic alcohols) were well separated with the separation factors (α) as high as 1.02～1.04 and resolutions (R) generally over 1.5. The method had been applied to determine the enantiomeric excess (ee) of samples produced by enantioselective synthetic reaction. The chiral recognition mechanism using the column (Rt-βDex^TM~cst, 30 m×0.25 mm ID) was discussed and believed that the reasonable hydrophobicity and the size of substituent of the sample molecules are important to reduce non-special interaction and form relatively stable asymmetric inclusion, which result in enantiomer separation.

Key words: PROPYNOL P, CYCLODEXTRIN, SEPARATION, GAS CHROMATOGRAPHY, MESOTOMY, ENANTIOMORPH