Chinese Journal of Organic Chemistry ›› 2022, Vol. 42 ›› Issue (6): 1687-1693.DOI: 10.6023/cjoc202112012 Previous Articles     Next Articles

ARTICLES

一种具有大斯托克斯位移和高光学稳定性的线粒体靶向近红外染料及其细胞成像应用

周红梅, 唐永和, 卢辉旭, 张倩, 林伟英*()   

  1. 广西大学化学化工学院 广西电化学能源材料重点实验室 广西大学光功能材料与化学生物学研究院 有色金属及材料加工新技术教育部重点实验室 广西有色金属及特色材料加工重点实验室 广西南宁 530004
  • 收稿日期:2021-12-06 修回日期:2022-02-17 发布日期:2022-03-21
  • 通讯作者: 林伟英
  • 基金资助:
    国家自然科学基金(21672083); 国家自然科学基金(21877048); 国家自然科学基金(22077048); 国家自然科学基金(22104019); 广西省自然科学基金(2021GXNSFDA075003); 广西省自然科学基金(AD21220061); 广西省自然科学基金(2019GXNSFBA245068); 广西大学启动基金(A3040051003)

A High Photostability Mitochondrial Targeted Near-Infrared Dye with Large Stokes Shift and Cell Imaging Application

Hongmei Zhou, Yonghe Tang, Huixu Lu, Qian Zhang, Weiying Lin()   

  1. Guangxi Key Laboratory of Electrochemical Energy Materials, Institute of Optical Materials and Chemical Biology, MOE Key Laboratory of New Processing Technology for Nonferrous Metals and Materials,Guangxi Key Laboratory of Processing for Non-ferrous Metals and Featured Materials,School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004
  • Received:2021-12-06 Revised:2022-02-17 Published:2022-03-21
  • Contact: Weiying Lin
  • Supported by:
    National Natural Science Foundation of China(21672083); National Natural Science Foundation of China(21877048); National Natural Science Foundation of China(22077048); National Natural Science Foundation of China(22104019); Natural Science Foundation of Guangxi Province(2021GXNSFDA075003); Natural Science Foundation of Guangxi Province(AD21220061); Natural Science Foundation of Guangxi Province(2019GXNSFBA245068); Startup Fund of Guangxi University(A3040051003)

Mitochondrial dysfunction is closely related to serious diseases such as Alzheimer’s disease (AD), hypertension, diabetes and neurodegenerative diseases. The development of a fluorescent dye that can be highly targeted to mitochondria is essential for studying the physiological functions of mitochondria. However, the Stokes shift of the mitochondrial dyes on the market is very small and is easily affected by the self-absorption effect. Herein, a new mitochondria targeted dye was designed, named as Mito-ql, based on the properties of mitochondrial membrane potential. The dye Mito-ql has excellent optical properties such as large Stokes shift (about 165 nm), good chemical and photo stability, and low cytotoxicity. In addition, the cell imaging experiments demonstrated that the dye Mito-ql has excellent mitochondrial localization ability, which is not affected by changes in mitochondrial membrane potential.

Key words: large Stokes shift, fluorescent imaging, mitochondria, fluorescent dye