Chinese Journal of Organic Chemistry ›› 2023, Vol. 43 ›› Issue (7): 2561-2566.DOI: 10.6023/cjoc202301012 Previous Articles     Next Articles

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金核霉素生物合成途径的体内研究和关键代谢物鉴定

王飞a, 金文兵b, 侯现锋b, 唐功利a,b,*(), 潘海学a,b,*()   

  1. a国科大杭州高等研究院化学与材料科学学院 杭州 310024
    b中国科学院上海有机化学研究所 上海 200032
  • 收稿日期:2022-11-11 修回日期:2023-02-24 发布日期:2023-03-17
  • 通讯作者: 唐功利, 潘海学
  • 基金资助:
    国家自然科学基金(32070065)

In vivo Studies on the Biosynthetic Pathway of Aureonuclemycin and Identification of Key Metabolites

Fei Wanga, Wenbing Jinb, Xianfeng Houb, Gongli Tanga,b(), Haixue Pana,b()   

  1. aSchool of Chemistry and Materials Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024
    bShanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032
  • Received:2022-11-11 Revised:2023-02-24 Published:2023-03-17
  • Contact: Gongli Tang, Haixue Pan
  • Supported by:
    National Natural Science Foundation of China(32070065)

On the basis of aureonuclemycin biosynthetic gene cluster obtained in the previous work, in-frame markerless deletion mutants of all genes were constructed, and the key genes that play a role in the biosynthesis of aureonuclemycin were identified. Two key aureonuclemycin-like compounds were successfully obtained from mutant and wild-type strains. According to the results of in vivo experiments, metabolite analysis and identification of key compounds, the possible biosynthetic pathway of aureonuclemycin was proposed.

Key words: nucleoside antibiotic, biosynthesis, aureonuclemycin