Chinese Journal of Organic Chemistry ›› 2020, Vol. 40 ›› Issue (10): 3467-3470.DOI: 10.6023/cjoc202005091 Previous Articles     Next Articles

Special Issue: 黄乃正院士七十华诞专辑


聂秋玥, 季珍瑜, 唐功利   

  1. 中国科学院上海有机化学研究所 上海 200032
  • 收稿日期:2020-05-30 修回日期:2020-07-03 发布日期:2020-07-23
  • 通讯作者: 唐功利
  • 基金资助:

Discovery of a Novel Spiro-Naphthoquinone Compound in Streptomyces aureus SP-371

Nie Qiuyue, Ji Zhenyu, Tang Gongli   

  1. Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032
  • Received:2020-05-30 Revised:2020-07-03 Published:2020-07-23
  • Supported by:
    Project supported by the National Key Research and Development Program of China (No. 2018YFA0901900).

By overexpressing the positive regulatory gene, the silent gene cluster tjh in Streptomyces aureus SP-371 was successfully activated. The mutant strain could produce compounds with two distinct core strucutures which are pentacyclic and tetracyclic structures. It was speculated that these two types of skeletons might be derived from the same intermediate through two different post-modified pathways. These two tailoring pathways were intercepted by the double gene knockout, and aureuspiro was successfully accumulated in the mutant. Aureuspiro is a new natural product with a novel spiro-naphthoquinone structure. Despite aureuspiro is not a true intermediate in the biosynthesis of final products, it could support the hypothesis about structure of the common intermediate.

Key words: genome mining, biosynthesis, type II polyketides natural product, spiro ring