### 高性能聚集诱导发光纳米探针用于肿瘤切除手术导航

1. 1 南开大学生命科学学院 药物化学生物学国家重点实验室 生物活性材料教育部重点实验室 天津 300071
• 投稿日期:2020-10-31 发布日期:2021-01-05
• 通讯作者: 丁丹
• 作者简介:
• 基金资助:
项目受国家自然科学基金(51961160730); 项目受国家自然科学基金(51873092); 项目受国家自然科学基金(81921004); 国家重点研发计划、中国-澳大利亚政府间国际科技创新合作项目(2017YFE0132200); 天津市杰出青年科学基金(19JCJQJC61200)

### High Performance Aggregation-Induced Emission Nanoprobes for Image-Guided Cancer Surgery

Heqi Gao1, Di Jiao1, Hanlin Ou1, Jingtian Zhang1, Dan Ding1,*()

1. 1 State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China
• Received:2020-10-31 Published:2021-01-05
• Contact: Dan Ding
• Supported by:
National Natural Science Foundation of China(51961160730); National Natural Science Foundation of China(51873092); National Natural Science Foundation of China(81921004); National Key R&D Program of China(Intergovernmental Cooperation Project)(2017YFE0132200); and the Tianjin Science Fund for Distinguished Young Scholars(19JCJQJC61200)

Far red/near infrared (FR/NIR) materials have attracted wide attention due to their great potential in various applications, particularly in bio-imaging. However, it is still a challenge to manufacture organic FR/NIR materials with quite high efficiency and long emission wavelength, owing to the dominance of non-radiative deactivation in the dissipation of absorbed light excitation energy when the electronic bandgap decreases. Herein, a series of donor-acceptor-donor (D-A-D) type compounds based on benzoselenidazole are developed through the regulation of molecular aggregation states by twisted conformation groups. In one hand, the compound TPE-DPA-Se (tetraphenylethylene-diphenylamine-benzoselenidazole) exhibits the best aggregation-induced emission (AIE) properties among these compounds. In another hand, the obtained TPE-DPA-Se showed over 150 nm Stokes shift, which can be used to avoid the interference between excitation and emission light, as well as the near-infrared emission spectrum away from the organism auto-fluorescence, which was beneficial for the bio-application. Next, density functional theory (DFT) calculation was carried out with Gaussian 09W program at B3LYP/6-31G** level to determine the highest occupied molecular orbital (HOMO), the lowest unoccupied molecular orbital (LUMO) distributions and the optimized structures of these compounds. Then TPE-DPA-Se was formulated into nanoparticles by nanoprecipitation method with an amphiphilic co-polymer 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N- [methoxy(polyethylene glycol)-2000] (MPEG2000-DSPE) as the doping matrix. TPE-DPA-Se exhibited excellent aqueous diameter stability in nanoparticle-state, as well as impressive luminescence in aqueous system with higher efficiency of up to 16.48%, which are suitable for many fields. To evaluate the biocompatibility of the TPE-DPA-Se NPs, we further carried out 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cytotoxicity assay. The result indicated their negligible toxicity. Encouraged by the excellent performance of these luminogens in nanoparticle state, a successful 4T1 cells imaging was demonstrated. Next, to set up the tumor-bearing mouse model, the luciferase-expressed 4T1 cancer cells were injected into the healthy mice via intraperitoneal injection. After about 7 days, the abdominal metastatic tumors were formed with many nodules in the abdominal cavity. The fluorescent image can merged with bioluminescence image completely and image-guided tumor resection is verified in this work, particularly for the micro-sized tumor of intraoperative detection.