化学学报 ›› 2004, Vol. 62 ›› Issue (22): 2277-2281. 上一篇    下一篇

研究论文

桑色素及其配合物与DNA作用的比较

王流芳1, 宋玉民2, 冯亚非3, 张歧1,4, 王印月1   

  1. 1. 兰州大学功能有机分子化学国家重点实验室, 兰州, 730000;
    2. 西北师范大学化学化工学院, 兰州, 730070;
    3. 湛江海洋大学理学院, 湛江, 524088;
    4.海南大学理工学院, 海口, 570228
  • 收稿日期:2004-11-03 修回日期:2004-07-16 出版日期:2004-11-28 发布日期:2014-02-17
  • 通讯作者: 王流芳,E-mail:wanglf@lzu.edu.cn;Fax:0931-8912582. E-mail:wanglf@lzu.edu.cn
  • 基金资助:
    甘肃省自然科学基金(No.ZSO21-A25-005-Z)及国家自然科学基金(No.50272027)资助项目.

Comparison between the Interactions of Morin and Its Complexes with DNA

WANG Liu-Fang1, SONG Yu-Min2, FENG Ya-Fei3, ZHANG Qi1,4, WANG Yin-Yue1   

  1. 1. State Key Laboratory of Functional Organic Chemistry, Lanzhou University, Lanzhou 730000;
    2. College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070;
    3. College of Science, Zhanjiang Ocean University, Zhanjiang 5240;
    4. College of Science and Engineering, Hainan University, Haikou 570228
  • Received:2004-11-03 Revised:2004-07-16 Online:2004-11-28 Published:2014-02-17

采用光度法、粘度法和电化学方法考察了桑色素及其配合物和DNA的作用.并对桑色素合铜(Ⅱ)、桑色素合锌(Ⅱ)、桑色素合钴(Ⅱ)、桑色素与DNA作用的方式进行了比较.Cu(Ⅱ), Zn(Ⅱ), Co(Ⅱ)桑色素配合物与DNA作用时的荧光光谱性质类似,不同于配体.钴(Ⅱ)配合物的电化学行为与铜(Ⅱ)配合物相似,不同于锌(Ⅱ)配合物.三种配合物的存在,都可引起DNA的粘度增加.但钴(Ⅱ)配合物对EB-DNA复合物的荧光光谱影响很小,而铜(Ⅱ)、锌(Ⅱ)配合物对EB-DNA复合物的荧光光谱影响较大.实验结果表明,与DNA之间的作用,钴(Ⅱ)配合物弱于铜(Ⅱ)、锌(Ⅱ)配合物.这可能是钴(Ⅱ)配合物的抗肿瘤活性低于与DNA嵌入结合的铜(Ⅱ)、锌(Ⅱ)配合物的原因之一.

关键词: 配合物, DNA, 作用模式

The interactions of Morin (2',3,4',5,7-pentahydroxyflavone) and its Zn(Ⅱ), Cu(Ⅱ), or Co(Ⅱ)-complex with calf thymus DNA have been studied by using spectral, viscometric and electrochemical methods. And there is a comparison between the interactions of Morin and its Co(Ⅱ), Cu(Ⅱ), or Zn(Ⅱ)-complexes with DNA. The complexes of Zn(Ⅱ) and Cu(Ⅱ) have the same spectral characteristics, which is different from that of Morin and Co(Ⅱ)-complex in the presence of DNA. The electrochemical behavior of Co(Ⅱ)-complex is the same as that of Cu(Ⅱ)-complex, but different from that of Zu(Ⅱ)-complex with the DNA addition. The relative viscosity of DNA increased with the addition of Zu(Ⅱ)-, Cu(Ⅱ)- or Co(Ⅱ)-complex. The results suggest that the Co(Ⅱ)-complex binds to DNA in a part-intercalating mode. This may be one of the reasons that the antitumour activity of Co(Ⅱ)-complex is weaker than that of Cu(Ⅱ) and Zn(Ⅱ)-complexes, which bind to DNA in intercalating mode.

Key words: complex, DNA, binding mode