化学学报 ›› 2009, Vol. 67 ›› Issue (21): 2481-2485. 上一篇    下一篇

研究论文

高温二维液相色谱系统的构建及其评价

李笃信a    魏远隆a    宋伦a    李彤b    杜一平a     张维冰*,a   

  1. (a华东理工大学化学与分子工程学院 上海 200237) (b大连依利特分析仪器有限公司 大连 116023) 5.  
  • 收稿日期:2009-03-06 修回日期:2009-06-03 出版日期:2009-11-14 发布日期:2009-07-13
  • 通讯作者: 张维冰 E-mail:weibingzhang@dicp.ac.cn
  • 基金资助:

    国家自然科学基金(批准号:20675083);“973”项目(2007CB914103)资助项目。

Construction and Evaluation of High Temperature Two-Dimensional Liquid Chromatography System

Li, Duxin    Wei, Yuanlong     Song, Luna       Li, Tong      Du, Yiping      Zhang, Weibing*,a   

  1. (a East China University of Science & Technology, School of Chemistry and Molecular Engineering, Shanghai 200237) (b Dalian Elite Analytical Instrument Co., Ltd., Dalian 116023)
  • Received:2009-03-06 Revised:2009-06-03 Online:2009-11-14 Published:2009-07-13

在二维液相色谱中, 第二维的分离速度是制约其发展的重要因素. 升高色谱柱温度可以有效降低流动相粘度, 加快溶质在两相间的传质速率, 有效加快分析速度. 以离子交换色谱法(WAX)为第一维分离模式和反相色谱法(RP)为第二维分离模式, 十通阀和两个捕集柱为接口, 通过将第二维色谱柱温度升高到80 ℃和提高流量到3 mL/min, 构建了高温WAX/RP二维液相色谱系统. 以4种标准蛋白的酶解物为样品评价系统的分离性能, 第一维共有33个馏分被捕集并导入到第二维分析, 高温二维液相色谱系统识别出187个色谱峰.

关键词: 高温, 二维液相色谱系统, 离子交换色谱/反相色谱, 多肽

The separation speed is very important to the development of two dimensional liquid chromatography. The viscosity of the mobile phase decreases and the analyte diffusivity increases at higher temperature. An ion exchange chromatography column was used as the first dimension mode and reversed phase chromatography as the second dimension mode, which were connected by the interface consisting of a ten-port valve and two trap columns. A high temperature two dimensional weak anion exchange (WAX)/ reversed phase (RP) liquid chromatography system was developed by the second dimensional column operated at 80 ℃ and higher flow rate up to 3 mL/min. A tryptic digest of 4 proteins was separated on this system. 33 fractions from the first dimension were introduced to the second dimension by the interface. 187 peaks were separated by the high temperature two dimensional liquid chromatography system.

Key words: high temperature, two-dimensional liquid chromatography, ion exchange/reversed phase liquid chromatography, peptide