化学学报 ›› 1997, Vol. 55 ›› Issue (3): 306-312. 上一篇    

研究论文

大豆糖蛋白的结构研究

郑建仙;耿立萍   

  1. 华南理工大学食品工程系
  • 出版日期:1997-03-15 发布日期:1997-03-15

Studies on the structure of soybean glycoprotein

ZHENG JIANXIAN;GENG LIPING   

  • Online:1997-03-15 Published:1997-03-15

应用NaClO2-HOAc提取结合层析柱提纯, 从大豆细胞壁物质(膳食纤维)中分离出PRF3纯组分。这是一种富含羟脯氨酸的糖蛋白, 本文对其结构特征作详尽剖析。结果表明, PRF3多糖部分的主链结构是由β(1→4)糖苷键连接的半乳聚糖,主链通过3-C分支点连接由α(1→3)键连的低聚阿拉伯糖侧链, 多糖与多肽之间通过HPr-Ara和Ser-Gal糖苷键连接在一起。

关键词: 次氯酸钠, 大豆, 结构分析, 糖蛋白, 醋酸钠, 膳食纤维

Pure fraction PRF3, hydroxyproline-rich glycoprotein, was isolated from soybean cell wall material (dietary fibre) through sodium chlorite-acetic acid (NaClO2-HOAc) extraction and chromatographic column purification. The results of intensive structural characterization showed that PRF3 contained β(1→4) linked galactan backbond which was branched at position 3-C with α(1→3) linked oligoarabinan as side chains, and the polypeptide was glycosidically linked through hydroxyprolinyl arabinosides and serinyl galactosides.

Key words: SODIUM HYPOCHLORITE, GLYCINE MAX, STRUCTURAL ANALYSIS, GLYCOPROTEIN

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