化学学报 ›› 2016, Vol. 74 ›› Issue (2): 149-154.DOI: 10.6023/A15090584 上一篇    下一篇

综述

糖蛋白/糖肽分离富集中的化学

张丽霞, 杜秀芳, 曾盈   

  1. 湖南师范大学化学化工学院化学生物学及中药分析教育部重点实验室 长沙 410081
  • 投稿日期:2015-09-05 发布日期:2015-11-25
  • 通讯作者: 曾盈 E-mail:yingzeng@hunnu.edu.cn
  • 基金资助:

    项目受国家自然科学基金青年项目(No.21205038)和湖南自然科学基金重点项目(No.13JJ2022)资助.

Chemistry in Separation and Enrichment of Glycoproteins/Glycopeptides

Zhang Lixia, Du Xiufang, Zeng Ying   

  1. Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, Ministry of Education of China, College of Chemistry and Chemical Engeneering, Hunan Normal University, Changsha 410081
  • Received:2015-09-05 Published:2015-11-25
  • Supported by:

    Project supported by the National Natural Science Fund of China (No. 21205038) and the Natural Science Foundation of Hunan Province, China (No. 13JJ2022).

糖蛋白在许多生命过程中有重要的作用.糖蛋白/糖肽在复杂生物样品中的低丰度以及糖链结构的微观不均一性使其分离富集成为糖蛋白质组学研究中的难点,本文将糖蛋白/糖肽分离富集过程中使用的化学方法根据所应用的化学反应或外源功能基团的种类分为:肼化学方法,氨化学方法,硼酸化学法, β-消除米氏加成化学法;并对各化学方法的原理和优缺点进行了描述和讨论.

关键词: 糖蛋白, 分离和富集, 糖蛋白组学, 化学

Glycoproteins play very important roles in many biological processes. The separation and enrichment of glycoproteins/glycopeptides is still considered a challenging task because of the low abundance and microheterogenity. This review introduces the chemistry method of separation and enrichment of glycoproteins and glycopeptides:hydrozide chemistry, amine chemistry, boronic acid chemistry, beta-elimination and Michael addition chemistry.

Key words: glycoprotein, separation and enrichment, glycoproteomics, chemistry