A novel label-free amperometric immunosensor for the determination of myeloperoxidase (MPO) based on poly-o-phenylenediamine (PoPD)-multi-wall carbon nanotubes (MWCNTs)-ionic liquid/ gold nanoparticles (nanogold) modified gold electrode (Au) was constructed. Firstly, a PoPD/MWNTs composite modified Au electrode was prepared in situ electropolymerization using ionic liquid as the supporting electrolyte. Then gold nanoparticles were adsorbed onto the electrode surface through amino-groups on the composite membrane for immobilization of MPO antibody via amine-Au affinity. The modification process was characterized by cyclic voltammetry (CV) and scanning electron microscope (SEM). The factors possibly influenced the performance of the proposed immunosensor were studied in detail. Under optimal conditions, the obtained immunosensor exhibited good electrochemical behavior to MPO in a linear range of 0.25 to 350 ng/mL (r＝0.9985), with a detection limit of 0.07 ng/mL. The immunosensor has advantages of good stability, regeneration, high sensitivity and specificity. Thus, the method can be used for clinical application of MPO analysis.
刘蓓, 逯岭松, 刘成桂, 谢国明. 基于PoPD-MWCNTs-离子液体/纳米金的髓过氧化物酶免疫传感器[J]. 化学学报, 2011, 69(04): 438-444.
LIU Bei, LU Ling-Song, LIU Cheng-Gui, XIE Guo-Ming. Immunosensor for Myeloperoxidase Based on PoPD-MWCNTs-Ionic Liquid/Nanogold Modified Au Electrode. Acta Chimica Sinica, 2011, 69(04): 438-444.