谷胱甘肽磁性分子印迹聚合物的制备及吸附性能研究

1. 兰州理工大学 省部共建有色金属先进加工与再利用国家重点实验室 材料科学与工程学院 甘肃 兰州 730050
• 投稿日期:2014-03-19 发布日期:2014-04-10
• 通讯作者: 杜雪岩 E-mail:duxy@lut.cn
• 基金资助:

项目受国家自然科学基金（No. 51061009）资助.

Preparation and Adsorption Property of Glutathione Magnetic Molecularly Imprinted Polymers

Wang Rujuan, Ma Yingxia, Lu Cuiping, Li Tao, Du Xueyan

1. State Key Laboratory of Advanced Processing and Recycling of Non-ferrous Metals, School of Materials Science & Engineering, Lanzhou University of Technology, Lanzhou 730050
• Received:2014-03-19 Published:2014-04-10
• Supported by:

Project supported by the National Natural Science Foundation of China (Grant No. 51061009).

The aim of this work was to produce a kind of magnetic molecular imprinted polymers (MMIPs) with core-shell structure and good magnetic separation performance. Compared with the preparation of traditional MIPs, a novel process was adopted to prepare MMIPs with good simplicity, flexibility and selectivity, in which Fe3O4 NPs were firstly prepared by a reverse microemulsion method, and subsequently directly added to the mixed solution to undergo the polymerization reaction without washing and drying. This method avoids availably the agglomeration and oxidation of Fe3O4 NPs. The MMIPs for glutathione (GSH) specific recognition (GSH-MMIPs), with core-shell structure, were prepared via one-step method polymerization, in which GSH as template molecule, N-vinylpyrrolidone (NVP) and acrylamide (AM) as functional monomers, N,N'-methylene bisacrylamide as crosslinker, H2O2-Vc as initiator and Fe3O4 nanoparticles (NPs) modified with γ-methacryloxypropyltrimethoxysilane (KH-570) as magnetic carrier. The as-prepared polymers were characterized by using of X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectroscopy and vibrating sample magnetometer (VSM). The results demonstrated that an imprinted polymer layer was successfully coated onto the surface of modified Fe3O4 NPs, resulting in a narrow diameter distribution and good magnetic responsibility. The specific adsorption equilibrium and selectivity were evaluated by batch rebinding studies. The Scatchard analysis showed that there were two kinds of binding sites in the GSH-MMIPs, the dissociation constants were 8.786×10-4 mol/L and 5.424×10-3 mol/L, and the corresponding maximum adsorption capacities of GSH onto GSH-MMIPs were 49.195 mg/g and 155.003 mg/g, respectively. The selectivity of the GSH-MMIPs was also evaluated to determine the rebinding capability of GSH and its analogues, compared with that of the magnetic non imprinted polymers (MNIPs) with similar chemical components. The GSH-MMIPs with high imprinting factor of 3.99 indicated that it was of good selectivity. This protocol was further employed for the extraction and separation of GSH from an aqueous solution.