化学学报 ›› 2014, Vol. 72 ›› Issue (5): 577-582.DOI: 10.6023/A14030196 上一篇    下一篇

研究论文

谷胱甘肽磁性分子印迹聚合物的制备及吸附性能研究

王茹娟, 马应霞, 路翠萍, 李涛, 杜雪岩   

  1. 兰州理工大学 省部共建有色金属先进加工与再利用国家重点实验室 材料科学与工程学院 甘肃 兰州 730050
  • 收稿日期:2014-03-19 出版日期:2014-05-14 发布日期:2014-04-10
  • 通讯作者: 杜雪岩 E-mail:duxy@lut.cn
  • 基金资助:

    项目受国家自然科学基金(No. 51061009)资助.

Preparation and Adsorption Property of Glutathione Magnetic Molecularly Imprinted Polymers

Wang Rujuan, Ma Yingxia, Lu Cuiping, Li Tao, Du Xueyan   

  1. State Key Laboratory of Advanced Processing and Recycling of Non-ferrous Metals, School of Materials Science & Engineering, Lanzhou University of Technology, Lanzhou 730050
  • Received:2014-03-19 Online:2014-05-14 Published:2014-04-10
  • Supported by:

    Project supported by the National Natural Science Foundation of China (Grant No. 51061009).

采用表面分子印迹技术,以谷胱甘肽(GSH)为模板分子,N-乙烯基吡咯烷酮(NVP)和丙烯酰胺(AM)为功能单体,NN’-亚甲基双丙烯酰胺(MBA)为交联剂,γ-(甲基丙烯酰氧)丙基三甲氧基硅烷(KH570)改性的Fe3O4纳米颗粒为磁性载体,制备了对GSH有特异识别性的磁性分子印迹聚合物(GSH-MMIPs). 利用X射线衍射仪(XRD)、透射电子显微镜(TEM)、红外光谱(FT-IR)和振动样品磁强计(VSM)对聚合物进行了表征,结果表明磁性载体表面成功地包覆了分子印迹聚合物薄层. 静态吸附平衡实验和Scatchard分析结果表明,GSH-MMIPs中存在两类不同的结合位点,平衡解离常数分别为8.786×10-4 mol/L和5.424×10-3 mol/L,最大吸附量分别为49.195 mg/g和155.003 mg/g. 与化学组成相同的磁性非印迹聚合物(GSH-MNIPs)相比,GSH-MMIPs对谷胱甘肽有较高的选择吸附性能.

关键词: 磁性纳米颗粒, 分子印迹聚合物, 谷胱甘肽, 吸附

The aim of this work was to produce a kind of magnetic molecular imprinted polymers (MMIPs) with core-shell structure and good magnetic separation performance. Compared with the preparation of traditional MIPs, a novel process was adopted to prepare MMIPs with good simplicity, flexibility and selectivity, in which Fe3O4 NPs were firstly prepared by a reverse microemulsion method, and subsequently directly added to the mixed solution to undergo the polymerization reaction without washing and drying. This method avoids availably the agglomeration and oxidation of Fe3O4 NPs. The MMIPs for glutathione (GSH) specific recognition (GSH-MMIPs), with core-shell structure, were prepared via one-step method polymerization, in which GSH as template molecule, N-vinylpyrrolidone (NVP) and acrylamide (AM) as functional monomers, N,N'-methylene bisacrylamide as crosslinker, H2O2-Vc as initiator and Fe3O4 nanoparticles (NPs) modified with γ-methacryloxypropyltrimethoxysilane (KH-570) as magnetic carrier. The as-prepared polymers were characterized by using of X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectroscopy and vibrating sample magnetometer (VSM). The results demonstrated that an imprinted polymer layer was successfully coated onto the surface of modified Fe3O4 NPs, resulting in a narrow diameter distribution and good magnetic responsibility. The specific adsorption equilibrium and selectivity were evaluated by batch rebinding studies. The Scatchard analysis showed that there were two kinds of binding sites in the GSH-MMIPs, the dissociation constants were 8.786×10-4 mol/L and 5.424×10-3 mol/L, and the corresponding maximum adsorption capacities of GSH onto GSH-MMIPs were 49.195 mg/g and 155.003 mg/g, respectively. The selectivity of the GSH-MMIPs was also evaluated to determine the rebinding capability of GSH and its analogues, compared with that of the magnetic non imprinted polymers (MNIPs) with similar chemical components. The GSH-MMIPs with high imprinting factor of 3.99 indicated that it was of good selectivity. This protocol was further employed for the extraction and separation of GSH from an aqueous solution.

Key words: magnetic nanoparticles, molecularly imprinted polymer, glutathione, adsorption