Acta Chimica Sinica ›› 2011, Vol. 69 ›› Issue (23): 2859-2864.DOI: 10.6023/A1104276 Previous Articles     Next Articles

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曾国平1,2, 向东山2, 李丽2, 何治柯*,2   

  1. (1湖北第二师范学院 武汉 430205)
    (2武汉大学化学与分子科学学院 生物医学分析化学教育部重点实验室 武汉 430072)
  • 投稿日期:2011-04-27 修回日期:2011-06-28 发布日期:2011-07-14
  • 通讯作者: 何治柯
  • 基金资助:


Resonance Rayleigh Scattering Spectra Method for Detection of Melamine in Milk with Gold Nanoparticles as Probe

Zeng Guoping1,2; Xiang Dongshan2; Li Li 2; He Zhike*,2   

  1. (1 Hubei University of Education, Wuhan 430205)
    (2 Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072)
  • Received:2011-04-27 Revised:2011-06-28 Published:2011-07-14

A new resonance Rayleigh scattering method was developed for the determination of melamine in milk using gold nanoparticles as spectral probe. Under certain conditions, melamine can form bigger aggregate with gold nanoparticles, which resulted in great enhancement of resonance Rayleigh scattering. Based on optimizing various parameters, the linear dynamic range, the Regression equation, the linear correlation and the detection limit were 3.3×10-8~4.7×10-7 mol/L, ΔIRRS=106.98C-28.279, R2=0.9971 and 2.7×10-8 mol/L, respectively. In this method, the gold nanoparticles do not need to be decorated. Only an amount of single-strand oligonucleotide (T10) need to be added to the system. The experiments showed that this developed method is practical, simple, sensitive and selective.

Key words: melamine, gold nanoparticles, resonance Rayleigh scattering