Acta Chimica Sinica ›› 2012, Vol. 70 ›› Issue (03): 284-290.DOI: 10.6023/A1109143 Previous Articles     Next Articles

Full Papers

基于UPLC/Q-TOF-MS 分析附子半夏配伍相反的物质基础

周思思a,b, 马增春b, 梁乾德b, 王宇光b, 谭洪玲b, 肖成荣b, 张伯礼c, 高月b   

  1. a 安徽医科大学研究生学院 合肥 230032;
    b 军事医学科学院放射与辐射医学研究所 北京 100850;
    c 天津中医药大学 天津 300193
  • 投稿日期:2011-09-14 修回日期:2011-11-08 发布日期:2011-11-30
  • 基金资助:

    国家重点基础研究发展计划(Nos. 2012CB518402, 2011CB505304)和北京市自然科学基金(No. 7112110)资助项目.

UPLC/Q-TOF-MS Based Chemical Profiling Approach to Evaluate Chemical Composition of Augmentation Toxicity in Combination of Radix Aconiti and Pinellia Praeparata

Zhou Sisia,b, Ma Zengchunb, Liang Qiandeb, Wang Yuguangb, Tan Honglingb, Xiao Chengrongb, Zhang Bolic, Gao Yueb   

  1. a Anhui Medical University, Hefei 230032;
    b Institute of Radiation Medicine Sciences, Academy of Military Medical Sciences, Beijing 100850;
    c Tianjin University of Traditional Chinese Medicine, Tianjin 300193
  • Received:2011-09-14 Revised:2011-11-08 Published:2011-11-30
  • Contact: 马增春, 高月;
  • Supported by:

    This work was supported by the National Basic Research Program of China (Nos. 2012CB518402, 2011CB505304) and the Natural Science Foundation of Beijing (No. 7112110).

In the present UPLC/Q-TOF-MS study, an ultra performance liquid chromatography coupled with time-of-fight mass spectrometry (UPLC/Q-TOFMS) based chemical profiling approach was used to evaluate chemical constitution between co-decoction and mixed decoction of radix aconiti and pinellia praeparata. Two different kinds of decoctions, namely radix aconiti and pinellia praeparata co-decoction: water extract of mixed two herbs, and radix aconiti and pinellia praeparata mixed decoction: mixed water extract of each individual herbs, were prepared. Batches of these two kinds of decoction samples were subjected to UPLC/Q-TOF-MS analysis, the datasets of tR-m/z pairs, ion intensities and sample codes were processed with orthogonal partial least squared discriminant analysis (OPLS-DA) to holistically compare the difference between these two kinds of decoction samples. The results of positive ion mode showed significant difference between two kinds of decoction samples, the content of hypaconitine, mesaconitine, aconitine, deoxyaconitine, 10-OH-mesaconitine, 10-OH-aconitine increased, while the content of mesaconine, dehydrated benzoylmesaconine, dehydrated benzoylhypaconine, benzoylaconine, benzoylhypaconine, 10- OH-benzoylmesaconine decreased in samples of co-decoction of radix aconiti and pinellia praeparata. The content of diester-diterpenoid alkaloids increased, while the content of monoester-diterpenoid alkaloids decreased, which may be basis of chemical composition in combination of radix aconiti and pinellia praeparata.

Key words: radix aconiti, pinellia praeparata, compatibility, LC-MS