Acta Chimica Sinica ›› 2013, Vol. 71 ›› Issue (03): 367-370.DOI: 10.6023/A12110962 Previous Articles     Next Articles

Article

基于裂开型核酸适配体的液晶生物传感检测三磷酸腺苷

吴超, 杨胜园, 吴朝阳, 沈国励, 俞汝勤   

  1. 湖南大学 化学生物传感与计量学国家重点实验室 长沙 410082
  • 收稿日期:2012-11-23 出版日期:2013-03-14 发布日期:2013-01-21
  • 通讯作者: 吴朝阳 E-mail:zywu@hnu.edu.cn
  • 基金资助:

    项目受国家自然科学基金(No.21277042)、新世纪优秀人才支持计划(No.NCET-11-0132)和湖南省科研条件创新专项重点项目(No.2011TT1004)资助.

Split Aptamer-Based Liquid Crystal Biosensor for ATP Assay

Wu Chao, Yang Shengyuan, Wu Zhaoyang, Shen Guoli, Yu Ruqin   

  1. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082
  • Received:2012-11-23 Online:2013-03-14 Published:2013-01-21
  • Supported by:

    Project supported by the National Natural Science Foundation of China (No. 21277042), the New Century Excellent Talents in University (No. 11-0132), and the Science and Technology Planning Project of Hunan Province (No. 2011TT1004).

A novel liquid crystal (LC) biosensor based on the recombination of split aptamer chip was developed for the detection of adenosine triphosphate (ATP). One glass slide of the LC cell is first modified with the TEA/DMOAP mixed self-assembled monolayer (SAM) to ensure the homeotropic alignment of LC molecules and the black background of LC cell optical image under the crossed polarized light. The single-strand ATP aptamer is split into two fragments in this method. One of which, as capture probe, is covalently immobilized on the SAM, while another is used as the detection probe. In the presence of ATP, those two fragments will be combined with each other and exhibit the same role as the ATP aptamer. This binding event leads to a great enhancement in the optical signal of the LC biosensor due to the space size from small to big, which can effectively disrupt the orientational arrangement of LCs, resulting in the corresponding changes of optical images under the crossed polarized light. But in the absence of ATP, those two fragments can not be combined with each other, and as a result, there is no orientational response of LCs and the optical image under the crossed polarized light is still black. So the LC-based imaging method has a sensitive and clear distinction between positive and negative results. On the basis of such an inhibition mechanism the LC biosensor can be used as an effective way to realize the detection of ATP. When the concentration of analyte ATP below a critical value (10 nmol/L), there is no clear change in the optical image. The results showed that the detection limit of ATP is 10 nmol/L. This study provides a simple and sensitive ATP LC biosensing approach and offers effective strategies for the development of small molecules LC biosensors.

Key words: liquid crystal, self-assembled film, biosensor, ATP, aptamer