有机化学 ›› 2017, Vol. 37 ›› Issue (3): 617-623.DOI: 10.6023/cjoc201610014 上一篇    下一篇

研究论文

水溶性罗丹明基Fe3+荧光探针及其在细胞成像中的应用

高勇a,b, 汪军a, 付妹a, 陈宏伟a, 方明章a   

  1. a 福建师范大学化学与化工学院 福州 350007;
    b 福建师范大学 福建省先进材料化工基础重点实验室 福州 350007
  • 收稿日期:2016-10-11 修回日期:2016-12-15 出版日期:2017-03-25 发布日期:2016-12-29
  • 通讯作者: 高勇,E-mail:gaoyong77@126.com E-mail:gaoyong77@126.com
  • 基金资助:

    福建省自然科学基金重点(No.2013H0019)资助项目.

A Water-Soluble Rhodamine-Based Fluorescent Probe for Fe3+ and Its Application in Live Cell Imaging

Gao Yonga,b, Wang Juna, Fu Meia, Chen Hongweia, Fang Mingzhanga   

  1. a College of Chemistry and Chemical Engineering, Fujian Normal University, Fuzhou 350007;
    b Fujian Provincial Key Laboratory of Advanced Materials Oriented Chemical Engineering, Fujian Normal University, Fuzhou 350007
  • Received:2016-10-11 Revised:2016-12-15 Online:2017-03-25 Published:2016-12-29
  • Contact: 10.6023/cjoc201610014 E-mail:gaoyong77@126.com
  • Supported by:

    Project supported by the Natural Science Foundation of Fujian Province (No. 2013H0019).

通过引入亲水性羧基,设计合成了一种水溶性罗丹明基荧光探针T1,其结构通过氢谱、碳谱、高分辨质谱等方法进行了表征.探针T1通过显著的荧光增强来识别Fe3+,并且有良好的选择性和抗干扰能力.该探针水溶性良好,可以在DMF/Tris-HCl(V:V=1:9,pH=7.4)的体系中使用.细胞成像实验显示该探针具有良好的细胞膜渗透能力,有可能用于生物细胞内Fe3+的检测.

关键词: 罗丹明, 水溶性, Fe3+, 荧光探针, 细胞成像

A rhodamine-based water-soluble fluorescent probe of Fe3+(T1) was designed and synthesized, in which a carboxy was linked to enhance its water solubility. Its structure was characterized by 1H NMR, 13C NMR and HRMS-ESI. The fluorescent probe showed remarkable "turn-on" fluorescent responses to Fe3+ with good selectivity over other competitive cations. It has a good water solubility that the recognition properties of the probe with Fe3+ ions can be investigated in DMF/Tris-HCl (V:V=1:9, pH=7.4). The fluorescence imaging experiments of Fe3+ in MCF-7 living cells demonstrated that the probe had a good cell-membrane permeability and it could be used in biological systems.

Key words: rhodamine, water solubility, Fe3+, fluorescent probe, live cell imaging