线粒体靶向的二氧化硫荧光探针研究进展

doi:10.6023/cjoc202210030

摘要/Abstract

二氧化硫(SO₂)与生命系统中的许多生理和病理过程有关, 过量的SO₂会导致一系列生理疾病. 因此, 有效地识别和检测SO₂具有显著的应用价值. 近年来, 已经报道了许多用于检测生物体系中SO₂的荧光探针. 与传统的检测方法相比, 荧光探针具有灵敏度高、无创检测及实时成像等优点, 更重要的是, 使用荧光探针可以在体外和体内对SO₂进行可视化检测. 根据不同的反应机理, 综述了近五年线粒体靶向的SO₂荧光探针的研究进展, 探讨了该领域面临的挑战和发展方向.

关键词: 线粒体, 荧光探针, 二氧化硫, 细胞成像

Content of abstract sulfur dioxide (SO₂) is associated with many physiological and pathological processes in living systems, and excessive SO₂ can lead to a series of physiological diseases. Therefore, effective identification and detection of SO₂ have significant application value. Recently, many fluorescent probes for the detection of SO₂ in biological systems have been reported. Compared with traditional detection methods, fluorescent probe has the advantages of high sensitivity, non-invasive detection and real-time imaging. More importantly, the use of fluorescent probes allows visual detection of SO₂ both in vitro and in vivo. Based on different reaction mechanism, the research progress of mitochondrial targeted SO₂ fluorescent probes in the past five years is reviewed, and the challenges and development directions in this field are discussed.

Key words: mitochondria, fluorescent probe, sulfur dioxide, cell imaging

引用此文

李宜芳, 王耀, 牛华伟, 陈秀金, 李兆周, 王永国. 线粒体靶向的二氧化硫荧光探针研究进展[J]. 有机化学, 2023, 43(6): 1952-1962.

Yifang Li, Yao Wang, Huawei Niu, Xiujin Chen, Zhaozhou Li, Yongguo Wang. Research Progress of Sulfur Dioxide Fluorescent Probe Targeting Mitochondria[J]. Chinese Journal of Organic Chemistry, 2023, 43(6): 1952-1962.

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Probe	LOD	Examination range	Response time
1	13.1 nmol/L	10~100 µmol/L	60 s
2	17 nmol/L	0~7 mmol/L	—^a
3	18.1 nmol/L	0~100 µmol/L	100 s
4	24 nmol/L	0~20 µmol/L	6 s
5	0.46 µmol/L	3.13~200 µmol/L	Within 30 min

Probe	LOD	Examination range	Response time
1	13.1 nmol/L	10~100 µmol/L	60 s
2	17 nmol/L	0~7 mmol/L	—^a
3	18.1 nmol/L	0~100 µmol/L	100 s
4	24 nmol/L	0~20 µmol/L	6 s
5	0.46 µmol/L	3.13~200 µmol/L	Within 30 min

Probe	LOD	Examination range	Response time
6	16 nmol/L	1.5~22.5 µmol/L	2 min
7	30 nmol/L	0~0.6 equiv.	—^a
8	15.6 nmol/L	0~2.5 µmol/L	13 min
9	0.37 µmol/L	0.01~0.15 mmol/L	—^a
10	85 nmol/L	0~3 equiv.	20 s
11	0.066 mmol/L/ 0.079 mmol/L	0~15 μmol/L/ 2~30 μmol/L	5 min
12	0.21 μmol/L	0~50 μmol/L	2 min
13	0.09 μmol/L	0~400 μmol/L	—^a
14	0.57 µmol/L	—^a	150 s
15	0.17 µmol/L	0~5 µmol/L	30 s
16	138 nmol/L	0~10 µmol/L	15 min
17	4.13 nmol/L	15~45 µmol/L	300 s

Probe	LOD	Examination range	Response time
6	16 nmol/L	1.5~22.5 µmol/L	2 min
7	30 nmol/L	0~0.6 equiv.	—^a
8	15.6 nmol/L	0~2.5 µmol/L	13 min
9	0.37 µmol/L	0.01~0.15 mmol/L	—^a
10	85 nmol/L	0~3 equiv.	20 s
11	0.066 mmol/L/ 0.079 mmol/L	0~15 μmol/L/ 2~30 μmol/L	5 min
12	0.21 μmol/L	0~50 μmol/L	2 min
13	0.09 μmol/L	0~400 μmol/L	—^a
14	0.57 µmol/L	—^a	150 s
15	0.17 µmol/L	0~5 µmol/L	30 s
16	138 nmol/L	0~10 µmol/L	15 min
17	4.13 nmol/L	15~45 µmol/L	300 s

Probe	LOD	Examination range	Response time
18	43 nmol/L	0~90 µmol/L	10 s
19	46 nmol/L	0~0.5 µmol/L	—^a
20	2 nmol/L	—^a	60 s
21	26.7 nmol/L	1~4 µmol/L	2 min
22	0.04 µmol/L	0~6 µmol/L	7 min
23	0.0126 µmol/L	—^a	35 s