有机化学 ›› 2018, Vol. 38 ›› Issue (4): 926-930.DOI: 10.6023/cjoc201708061 上一篇    下一篇

研究论文

一种Hg2+荧光探针的合成及其在活细胞成像中的应用

张敏a, 肖慧丰a, 韩志湘b, 仰榴青a, 吴向阳b   

  1. a 江苏大学化学化工学院 镇江 212013;
    b 江苏大学环境与安全工程学院 镇江 212013
  • 收稿日期:2017-08-30 修回日期:2017-11-09 出版日期:2018-04-24 发布日期:2017-12-01
  • 通讯作者: 吴向阳 E-mail:wuxy@ujs.edu.cn
  • 基金资助:

    江苏省自然科学基金(No.BK20140536)资助项目.

A Hg2+-Selective Fluorescence Probe: Synthesis and Application in Aqueous Solution and Living Cell Imaging

Zhang Mina, Xiao Huifenga, Han Zhixiangb, Yang Liuqinga, Wu Xiangyangb   

  1. a School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013;
    b School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013
  • Received:2017-08-30 Revised:2017-11-09 Online:2018-04-24 Published:2017-12-01
  • Contact: 10.6023/cjoc201708061 E-mail:wuxy@ujs.edu.cn
  • Supported by:

    Project supported by the Natural Science Foundation of Jiangsu Province (No. BK20140536).

设计合成了一个新的罗丹明席夫碱类荧光探针1,其结构经1H NMR、13C NMR、MS和X射线单晶衍射的验证.通过紫外-可见光谱法和荧光光谱法研究了探针1在乙醇-水(V/V=7/3,HEPES 10 mmol/L,pH=7.0)中对Hg2+的识别性能.探针1通过显著的荧光增强来识别Hg2+,并具有良好的选择性和抗干扰能力.通过Job's plot和MS证明了探针1和Hg2+形成1:1的配合物.探针1的荧光强度与Hg2+浓度(0~50 μmol/L)呈良好的线性关系,可以定量地检测该范围内的Hg2+.在MGC-803活细胞中的荧光显微成像表明,探针1可检测生物体内的Hg2+.

关键词: 罗丹明B, 合成, 荧光探针, Hg2+, 生物成像

A novel rhodamine B Schiff-base fluorescence probe 1 was synthesized and characterized by 1H NMR, 13C NMR, MS and single-crystal X-ray analysis. The recognition properties of the probe 1 with Hg2+ ions were investigated in ethanol-water (V/V=7/3, HEPES 10 mmol/L, pH=7.0) by the UV-Vis spectrophotometry and the fluorescence spectrophotometry. The probe 1 showed remarkable "turn-on" fluorescent responses to Hg2+ with good selectivity over the other metal ions examined. The 1:1 binding model of probe 1 with Hg2+ was established by Job's plot and MS spectra. The probe can be applied to the quantitative analysis of Hg2+ with a linear range covering from 0 to 50 μmol/L. The fluorescence imaging experiments in MGC-803 living cells demonstrated that 1 could be used in biological systems to monitor Hg2+.

Key words: rhodamine B, synthesis, fluorescent probe, Hg2+, bioimaging