有机化学 ›› 2019, Vol. 39 ›› Issue (5): 1483-1488.DOI: 10.6023/cjoc201811034 上一篇    下一篇

所属专题: 荧光探针-生物传感合辑 有机超分子化学合辑

研究简报

基于荧光共振能量转移效应的比例计量型水合肼荧光探针的设计、合成及其应用研究

杨滋琦a, 刘兴坤b, 姜鲁南b, 王美a   

  1. a 河北大学化学与环境科学学院 保定 071002;
    b 河北大学药学院 保定 071002
  • 收稿日期:2018-11-28 修回日期:2019-01-07 出版日期:2019-05-25 发布日期:2019-01-18
  • 通讯作者: 王美 E-mail:wangmarian@163.com
  • 基金资助:

    国家自然科学基金(No.51578067)、河北省自然科学基金(No.2018201234)、河北省高等学校科学技术研究项目(No.QN2017015)和保定市科学研究与发展计划(Nos.16zg031,18ZF315)资助项目.

Design, Synthesis and Application of Fluorescence Resonance Energy Transfer-Based Ratiometric Hydrazine Fluorescent Probe

Yang Ziqia, Liu Xingkunb, Jiang Lu'nanb, Wang Meia   

  1. a College of Chemistry and Environmental Science, Hebei University, Baoding 071002;
    b College of Pharmacy, Hebei University, Baoding 071002
  • Received:2018-11-28 Revised:2019-01-07 Online:2019-05-25 Published:2019-01-18
  • Contact: 10.6023/cjoc201811034 E-mail:wangmarian@163.com
  • Supported by:

    Project supported by the National Natural Scinec Foundation of China (No. 51578067), the Natural Science Foundation of Hebei Provience (No. 2018201234), the Colleges and Universities Science Technology Research Project of Hebei Province (No. QN2017015) and the Science Technology Research and Development Guidance Programme Project of Baoding City (Nos. 16zg031, 18ZF315).

水合肼(N2H4)是一种高毒性水溶生化试剂,具有致癌、致畸、致突变的能力.为了准确检测环境及生命体内N2H4浓度,基于荧光共振能量转移(FRET)原理,设计合成了两个双发射比例计量型探针(FRET-1/2),探针结构均经核磁共振氢谱、碳谱和高分辨质谱确证.研究结果表明,两个探针对N2H4均表现出较高的选择性和良好的灵敏性,可用于检测水样中N2H4浓度.

关键词: 水合肼, 荧光共振能量转移(FRET), 比例计量, 荧光探针

Hydrazine (N2H4) is a highly toxic biochemical reagent with the capability of mutagenic, teratogenic and carcinogenic. For accurately monitoring the concentration of N2H4 in the environment and life, two FRET (fluorescence resonance energy transfer)-based dual-emissive ratiometric fluorescent probes (FRET-1/2) were designed and synthesized. The structures of both probes were charactered by 1H NMR, 13C NMR and HRMS. The results prove that both probes exhibit good selectivity and sensitivity to N2H4 and can be used for detecting N2H4 in water samples.

Key words: hydrazine, fluorescence resonance energy transfer (FRET), ratiometric, fluorescent probe