有机化学 ›› 2019, Vol. 39 ›› Issue (9): 2467-2484.DOI: 10.6023/cjoc201901024 上一篇    下一篇

综述与进展

生物小分子成像用双光子荧光探针

黄池宝a,b, 陈会a, 李福琴a, 安思雅a   

  1. a 遵义师范学院化学化工学院 遵义 563002;
    b 韶关大学农业科学与工程学院 韶关 512005
  • 收稿日期:2019-01-17 修回日期:2019-02-24 出版日期:2019-09-25 发布日期:2019-04-08
  • 通讯作者: 黄池宝 E-mail:huangchibao@163.com
  • 基金资助:

    贵州省高层次创新型人才培养计划项目——"百"层次人才(No.[2016]5683)、国家自然科学基金(No.21562050)、贵州省第八批科技创新人才团队建设专项基金[No.(2015)4007]、贵州省科学技术基金(No.J[2015]2146)、贵州省教育厅自然科学重点研究(No.KY[2014]296)和贵州省教育厅省级本科教学工程建设(No.KY[2014]JXGChcb)资助项目.

Two-Photon Fluorescence Probes for Small Biomolecules Imaging

Huang Chibaoa,b, Chen Huia, Li Fuqina, An Siyaa   

  1. a Chemistry and Chemical Engineering College, Zunyi Normal University, Zunyi 563002;
    b College of Agricultural Science and Engineering, Shaoguan University, Shaoguan 512005
  • Received:2019-01-17 Revised:2019-02-24 Online:2019-09-25 Published:2019-04-08
  • Contact: 10.6023/cjoc201901024 E-mail:huangchibao@163.com
  • Supported by:

    Project supported by the Guizhou Province High-level Innovative Talents Training Project-Hundred Talents Program (No.[2016]5683), the National Natural Science Foundation of China (No. 21562050), the Special Fund Project of the Construction of the Eighth Batch of Scientific and Technological Innovation Talent Team in Guizhou Province[No. (2015)4007], the Guizhou Science and Technology Fund Project (No. J[2015]2146), the Key Project of Education Department of Guizhou Province (No. KY[2014]296) and the Teaching Contents and Curriculum System Reform Project of Higher Education in Guizhou Province (No. KY[2014] JXGChcb).

生物体内活性小分子(生物小分子),不仅数量多,涉及到无机小分子,如SO2,H2S,NO与CO等,而更多的是有机小分子,如单煻、低聚糖、激素、辅酶、甘油、刺激因子、调节因子和维他命等;而且在病理、生理等方面有着至关重要的功能,发挥着举足轻重的作用,直接影响身体健康与疾病的发生.因此,对生物体内小分子的实时观测和监控是十分必要的,而双光子荧光探针正是实现这一目标的必选锐器.双光子荧光探针的定点激发、高横向与纵向分辨率、无光漂白性、无光致毒性和组织深度成像等诸多优点而彰显其无与伦比的优越性,可以很好地对细胞或组织内的生物小分子进行实时动态三维观测和监控.综述了近5年来相继开发的CO、单糖(葡萄糖、β-半乳糖苷酶)、SO2、硫化氢、NO、过氧(硫)化物、硫醇/硫酚、1O2、甲醛、HNO、HClO、O2·-和ONOO-等双光子荧光探针,系统全面地剖析了这些双光子荧光探针的传感机制,并展望了生物小分子显微用双光子荧光探针的发展与愿景.

关键词: 生物小分子, 荧光探针, 传感机制, 荧光团, 受体, 双光子显微

Small molecules (biological small molecules) in vivo are not only in a large number, involving inorganic small molecules, such as SO2, H2S, NO and CO, and more organic small molecules, such as monosaccharides, oligosaccharides, hormones, coenzymes, glycerol, stimulating factors, regulatory factors, Vitamins, etc., moreover, play an important role in pathology, physiology, and so on. Therefore, it is necessary to observe and monitor small molecules in vivo in real time, and the two-photon fluorescence probe is a necessary tool to achieve this goal. The advantages of two-photon fluorescence probe, such as fixed target (very small dot) excitation, high horizontal and vertical resolution, no photobleaching, no phototoxicity and deep imaging in tissues, and so on, demonstrate its unparalleled superiority. It can be used for dynamic 3D observation and monitoring of biological small molecules in cells or tissues. In this paper, CO, monosaccharide (glucose, β-galactosidase), SO2, H2S, NO, peroxy (sulfur) compounds, mercaptan/thiophenol, 1O2, formaldehyde, HNO, HclO, O2·- and ONOO- two-photon fluorescence probes which have been developed in recent 5 years were reviewed. The sensing mechanisms of these two-photon fluorescence probes were systematically analyzed, and the development and prospect of two-photon fluorescence probes for small biomolecules are prospected.

Key words: biological small molecule, fluorescent probe, sensing mechanism, fluorophore, receptor, two-photon bioimaging