有机化学 ›› 2020, Vol. 40 ›› Issue (11): 3794-3801.DOI: 10.6023/cjoc202005046 上一篇    下一篇

综述与进展

超快速和二维液相色谱手性分离

许瑶, 康经武   

  1. 中国科学院上海有机化学研究所 生命有机化学国家重点实验室 上海 200032
  • 收稿日期:2020-05-18 修回日期:2020-06-29 发布日期:2020-07-17
  • 通讯作者: 康经武 E-mail:jingwu.kang@sioc.ac.cn
  • 基金资助:
    国家自然科学基金(Nos.21775158,21375140,21175146)资助项目.

Chiral Separation by Ultrafast and Two-Dimensional Liquid Chromatography

Xu Yao, Kang Jingwu   

  1. State Key Laboratory of Bioorganic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Science, Shanghai 200032
  • Received:2020-05-18 Revised:2020-06-29 Published:2020-07-17
  • Supported by:
    Project supported by the National Natural Science Foundation of China (Nos. 21775158, 21375140, 21175146).

虽然以多糖类手性固定相为标志的手性分离技术已经趋于成熟,但是复杂样品的手性分离仍然面临许多挑战.为此,近年来二维液相色谱手性分离得到了快速发展.二维液相色谱需要第二维色谱分离要足够快,因此又需要发展超快速液相色谱分离技术.超快速液相色谱和二维液相色谱的发展不仅仅提高了手性分离效率,同时也迎合了高通量有机合成技术发展的需求.总结了近年来超快速液相色谱和二维液相色谱在手性分析方面的研究进展,重点讨论了超快速液相色谱手性填料技术的发展和在解决复杂手性样品分析时的策略和应用,最后对超快速和二维液相在高通量有机合成中的应用前景做了展望.

关键词: 超快速液相色谱, 二维液相色谱, 手性分离, 高通量分离

Although the chiral separation techniques represented by the polysaccharides-based chiral stationary phases are almost matured, the chiral separation for complex samples remains a challenge. To this end, two dimensional liquid chromatography has been developed rapidly. Recently, to achieve the chiral separation with two dimensional liquid chromatoraphy, the development of the ultrafast liquid chromatography has caused considerable attention. The development of ultrafast liquid chromatography and two-dimensional liquid chromatography also caters to the development of high-throughput organic synthesis technology. In this review the recent progress in the chiral separation by the ultrafast and two-dimensional liquid chromatography is summarized. This review focuses on the progress in the techniques of the chiral packing materials for ultrafast chromatography, the chiral separation strategy and its application in complex samples. Finally, the application perspective of ultra-fast and two-dimensional liquid phase in high-throughput organic synthesis techniques is prospected.

Key words: ultrafast liquid chromatography, two-dimensional liquid chromatography, chiral separation, high-throughput separation