有机化学 ›› 2015, Vol. 35 ›› Issue (1): 175-180.DOI: 10.6023/cjoc201407018 上一篇    下一篇

研究论文

基于罗丹明B新型Fe3+荧光探针的合成及其性能研究

苏娜, 杨美盼, 孟文斐, 杨秉勤   

  1. 合成与天然功能分子化学教育部重点实验室 西北大学化学与材料科学学院 西安 710069
  • 收稿日期:2014-07-10 修回日期:2014-08-22 出版日期:2015-01-25 发布日期:2014-09-12
  • 通讯作者: 杨秉勤 E-mail:yangbq@nwu.edu.cn
  • 基金资助:

    国家自然科学基金(No. 21172178)资助项目.

Synthesis and Properties Characterization of Novel Fe3+ Fluorescent Probe Based on Rhodamine B

Su Na, Yang Meipan, Meng Wenfei, Yang Bingqin   

  1. Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an 710069
  • Received:2014-07-10 Revised:2014-08-22 Online:2015-01-25 Published:2014-09-12
  • Supported by:

    Project supported by the National Natural Science Foundation of China (No. 21172178)

以罗丹明B与2,6-二氨基吡啶缩合反应得到新型罗丹明B衍生物R1, 其结构经1H NMR, 13C NMR, IR, HRMS-ESI, X-Ray单晶衍射及元素分析表征. 研究发现化合物R1作为荧光探针能选择性比色地识别Fe3+, 且受常见金属离子的干扰较小. 探针R1的荧光强度与Fe3+浓度(4×10-6~1.6×10-5 mol/L)呈较好的线性关系, 并在HepG2活细胞中实现了Fe3+的成像.

关键词: 罗丹明B, 荧光探针, 铁离子, 识别, 荧光强度, HepG2活细胞

A novel rhodamine B derivative R1 was synthesized facilely by the condensation reaction of rhodamine B and 2,6-diaminopyridine, and its structure was characterized by 1H NMR, 13C NMR, IR, HRMS-ESI, X-ray techniques and elemental analysis. The results showed that the compound R1 exhibited the selective colorimetric to Fe3+, and other metal ions did not interfere with its recognition. The fluorescence intensity of probe R1 and Fe3+ concentration (4×10-6~1.6×10-5 mol/L) showed a good linear relationship. What's more, probe R1 has been applied to image intracellular Fe3+ in living HepG2 cells.

Key words: rhodamine B, fluorescent probe, iron ion, recognition, fluorescence intensity, HepG2 living cells