Chin. J. Org. Chem. ›› 2016, Vol. 36 ›› Issue (7): 1696-1699.DOI: 10.6023/cjoc201602016 Previous Articles     Next Articles



亢文佳a, 吴晟b, 华会明a, 潘海学b, 唐功利b   

  1. a. 沈阳药科大学中药学院 基于靶点的药物设计与研究教育部重点实验室 沈阳 110016;
    b. 中国科学院上海有机化学研究所 上海 200032
  • 收稿日期:2016-02-18 修回日期:2016-03-01 发布日期:2016-03-18
  • 通讯作者: 华会明, 唐功利;
  • 基金资助:


Discovery of a New Pyrazinone Natural Product by Genome Mining

Kang Wen-Jiaa, Wu Shengb, Hua Huiminga, Pan Hai-Xueb, Tang Gong-Lib   

  1. a. Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016;
    b. Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032
  • Received:2016-02-18 Revised:2016-03-01 Published:2016-03-18
  • Supported by:

    Project supported by the National Natural Science Foundation of China (Nos. 81373307 & 81473124).

The genomic sequences of Streptomyces sp. TP-A0365 were scanned using a secondary metabolite genome mining approach. A new nonribosomal peptide synthetase (NRPS) gene responsible for the biosynthesis of an unknown pyrazinone compound was found. The biosynthetic pathway of related compound was disrupted by inactivating the NRPS gene. Comparing the fermentation products of the mutant strain and the original strain (TG1301 derived from Streptomyces sp. TP-A0365), we caught compound 1 which disappeared in the mutant strain. Compound 1 was isolated from 20 L fermentation cultures of the original strain and its structure was established as 3-isopropyl-7,8-dihydropyrrolo[1,2-a]pyrazin-4(6H)-one, a new pyrazinone compound designated as provalin

Key words: pyrazinone, Streptomyces, genome mining, biosynthesis