To develop novel pesticide lead compounds with high activity，low toxicity and low residue，24 novel 5-(4-hydroxybenzyl)-2,4-imidazolidinedione (hydantoin) esters were synthesized with cyclization and esterification using L-tyrosine as raw material by simulating the structure of natural inhibitor against AdSS synthetase，and structurally confirmed by m.p.，IR，1H NMR and elemental analysis。X-ray diffraction analysis of 6m was studies as C18H16N2O5，Mr＝340.33，monoclinic，space group P2(1)/c，a＝0.86071(17) nm， b＝1.1167(2) nm，c＝0.93826(2) nm，β＝115.10(3)°，V＝0.8166(3) nm3，Dc＝1.384 g/cm3，Z＝2，F(000)＝356，m(Mo Kα)＝0.103 mm－1，final R＝0.0359，wR＝0.0998。The preliminary bioassay results indicated that some of these compounds had certain inhibition activities against Triticum aestivum and Arabidopsis thaliana，while some of them could promote the growth of Zea mays at the concentration of 200 μg/mL.

Key words: AdSS inhibitor, 5-(4-hydroxybenzyl)-2,4-imidazolidinedione ester, biological activity