Chin. J. Org. Chem. ›› 2017, Vol. 37 ›› Issue (2): 356-374.DOI: 10.6023/cjoc201607004 Previous Articles     Next Articles

REVIEW

溶酶体荧光探针研究新进展

李美含, 王宇童, 刘广建, 吕海娟, 邢国文   

  1. 北京师范大学化学学院 北京 100875
  • 收稿日期:2016-07-02 修回日期:2016-09-16 出版日期:2017-02-25 发布日期:2016-10-08
  • 通讯作者: 邢国文 E-mail:gwxing@bnu.edu.cn
  • 基金资助:

    国家自然科学基金(No.21272027)资助项目.

Recent Progress on Lysosome-Targetable Fluorescent Probes

Li Meihan, Wang Yutong, Liu Guangjian, Lü Haijuan, Xing Guowen   

  1. College of Chemistry, Beijing Normal University, Beijing 100875
  • Received:2016-07-02 Revised:2016-09-16 Online:2017-02-25 Published:2016-10-08
  • Supported by:

    Project supported by the National Natural Science Foundation of China (No. 21272027).

Lysosomes are vital in many physiological processes such as metabolism, membrane repair, cell apoptosis, etc. To have lysosomes visualized and reactive small molecules (RSMs) detected are of great significance on the understanding of some intracellular dynamic procedures as well as the therapy of related deseases. In the past few years, many lysosome-targeting RSMs-sensing fluorescent probes are reported, including protons, reduction species, oxidation species, metal cations, anions, enzymes and some physical properties such as pH, viscosity and temperature. The mechanism of targeting lysosomes can be classified into three types: (1) based on the acidic physiological environments in lysosomes, (2) based on the pathway of substance metabolism, especially the endocytosis of materials, and (3) based on the specific membrane proteins and hydrolases in lysosomes. The recently reported lysosomal fluorescent probes were assorted, summarized and reviewed in this work. The bright prospects of these probes in the application of preliminary diagnosis and therapy of some diseases were also discussed.

Key words: lysosomes, fluorescent probes, cell apoptosis, reactive small molecules, cellular microenvironment