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化学学报
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化学学报 ›› 1996, Vol. 54 ›› Issue (4): 413-416. 上一篇    

研究论文

用高效毛细管电泳测定二(2'-脱氧-2'-氟)嘧啶核糖核苷酸对蛇毒磷酸二酯酶的稳定性

王政甫;陈耀全   

  1. 中国科学院上海有机化学研究所;中国科学院生命有机化学国家重点实验室
  • 发布日期:1996-04-15

High performance capillary electrophoresis as monitor for determination of stability of di(2'-deoxy-2'-fluoro) pyrimidinenucleosidephosphates to snake venom phosphodiesterase

WANG ZHENGFU;CHEN YAOQUAN   

  • Published:1996-04-15

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337

被引次数

4 | 1

本文报道用高效毛细管电泳测定蛇毒磷酸二酯酶催化的二(2'-脱氧-2'-氟)嘧啶核糖核苷酸的水解速度。高效毛细管电泳用于这个目的有分辨率高、样品需要量'、使用不含有机溶剂的缓冲液和操作方便等优点。用来测定具有中等反应速度的反应是很方便的。

关键词: 稳定性, 嘧啶 P, 催化, 核糖核苷酸, 高效毛细管电泳, 蛇毒磷酸二酯

High performance capillary electrophoresis (HPCE) has been used as monitor for the determination of stability of di(2'-deoxy-2'-fluoro) cytidine phosphate and di(2'-deoxy-2'-fluoro) uridine phosphate to snake venom phosphodiesterase (SVPDE). This seems to be a good method for monitoring the enzymatic reactions of moderate reaction rate.

Key words: STABILITY, PYRIMIDINE P, CATALYSIS, RIBONUCLEOTIDE

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