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化学学报
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化学学报 ›› 2008, Vol. 66 ›› Issue (1): 108-111. 上一篇    下一篇

研究论文

苏云金芽孢杆菌毒素Cry1Aa、Cry2Aa、Cry3Aa和Cry4Aa结构的计算机对比分析

赵新民1,2，夏立秋*,1，王发祥1，丁学知1，单世平1，张友明1   

  1. (1湖南师范大学生命科学学院 微生物分子生物学湖南省重点实验室 长沙 410081)
    (2湖南城市学院化学与环境工程系 益阳 413000)
  • 投稿日期:2007-05-28 修回日期:2007-08-20 发布日期:2008-01-14
  • 通讯作者: 夏立秋

Comparison and Analysis of Cry1Aa, Cry2Aa, Cry3Aa and Cry4Aa of Bacillus thuringiensis Toxins with Computer

ZHAO Xin-Min1,2 XIA Li-Qiu*,1 WANG Fa-Xiang1 DING Xue-Zhi1 SHAN Shi-Ping1 ZHANG You-Ming1   

  1. (1 Key Laboratory for Molecular Biology of Microorganism of Hunan Province, College of Life Science, Hunan Normal University, Changsha 410081)
    (2 Department of Chemistry and Environmental Engineering, Hunan City University, Yiyang 413000)
  • Received:2007-05-28 Revised:2007-08-20 Published:2008-01-14
  • Contact: XIA Li-Qiu

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运用生物信息学软件对苏云金芽孢杆菌毒素Cry1Aa、Cry2Aa、Cry3Aa和Cry4Aa的 序列和基本参数、二级结构、三级结构、跨膜区和表面电势进行了预测比较。它们在一级 结构上有较大差异，但二级结构和跨膜区相似，三级结构中各毒素的结构域Ⅰ之间基本相 似，结构域Ⅱ之间差异较大，其中Cry2Aa为差异最大成员。4种毒素的表面电势分布不同。 毒素之间的结构相似性和差异性与其作用机理和杀虫特异性有关。

关键词: 苏云金芽孢杆菌, 毒素, 结构, 生物信息学, 计算机

The primary parameters, secondary and tertiary structures and transmembrane regions were compared in bioinformatics. Considerable differences were found among the four toxins compared each other in primary structure, and many similarities in secondary structure and transmembrane regions. The structural diversity is smallest in domain I, domain II is the most divergent domain, and Cry2Aa is the most divergent member. The surface potential over the four toxins were differently. Those structural diversity and similarity may be involved in determining action mode and insect specificity.

Key words: Bacillus thuringiensis, toxin, structure, bioinformatics, computer