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化学学报
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化学学报 ›› 2009, Vol. 67 ›› Issue (10): 1087-1092. 上一篇    下一篇

研究论文

CdSe/ZnS量子点探针用于检测猪链球菌2型溶菌酶释放蛋白(MRP)抗原的新方法研究

武红敏a 韩鹤友*,a 金梅林b 张安定b

  

  1. (a华中农业大学理学院 农业微生物学国家重点实验室 武汉 430070)
    (b华中农业大学动物科技学院 农业微生物学国家重点实验室 武汉 430070)

  • 投稿日期:2008-07-04 修回日期:2008-12-20 发布日期:2009-05-28
  • 通讯作者: 韩鹤友

A Novel Method to Detect Muramidase Released Protein(MRP) Antigen of Streptococcus sui Serotype 2 Based on CdSe/ZnS Quantum Dot Probe

Wu, Hongmin a Han, Heyou *,a Jin, Meilin b Zhang, Anding b   

  1. (a College of Science, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070)
    (b College of Veterinary Medicine, State Key Laboratory of Agricultural Microbiology,
    Huazhong Agricultural University, Wuhan 430070)
  • Received:2008-07-04 Revised:2008-12-20 Published:2009-05-28
  • Contact: Han, Heyou

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被引次数

39 | 3

合成了高发光效率的CdSe/ZnS量子点并制备了CdSe/ZnS量子点-溶菌酶释放蛋白(MRP)抗体探针, 利用凝胶电泳和分子光谱法研究了MRP抗体与CdSe/ZnS量子点的结合机理. 荧光光谱法优化了CdSe/ZnS量子点-MRP抗体探针制备的影响因素, 建立了一种测定MRP抗原的新方法, 其线性范围为5.0×10－8～1.5×10－6 mol/L, 线性相关系数为0.9976, 检测限为 1.9×10－8 mol/L.

关键词: 猪链球菌2型, 溶菌酶释放蛋白(MRP), CdSe/ZnS量子点探针, 检测

High-quality CdSe/ZnS quantum dot and the CdSe/ZnS quantum dot-muramidase released protein (MRP) antibody probe were prepared, and the conjugation mechanism was studied according to the results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and molecular spectrometry. The factors influencing the preparation of CdSe/ZnS quantum dot-MRP antibody probe were optimized by fluorescence spectrometry. Under the optimum conditions, the probe showed good sensitivity to MRP antigen. The calibration graph was linear in the range from 5.0×10－8 to 1.5×10－6 mol/L of MRP antigen concentration, with a correlation coefficient of 0.9976 and a detection limit of 1.9×10－8 mol/L.

Key words: Streptococcus Suis serotype 2, muramidase released protein (MRP), CdSe/ZnS quantum dot probe, detection