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化学学报
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化学学报 ›› 2006, Vol. 64 ›› Issue (22): 2296-2300. 上一篇    

研究简报

结合电生孔技术的抗体-纳米TiO2靶向光敏杀癌细胞

许娟1, 赵尧敏1, 陈春妹2, 孙毅1, 刘国元2, 严曼明1, 江志裕*,1   

  1. (1复旦大学化学系 分子催化与新材料重点实验室 上海 200433)
    (2复旦大学遗传学研究所 上海 200433)
  • 投稿日期:2005-12-23 修回日期:2006-05-18 发布日期:2006-11-28
  • 通讯作者: 江志裕

Oriented Photo-killing Tumor Using Antibody-nano-TiO2 Conjugates and Electroporation Methods

XU Juan1; ZHAO Yao-Min1; CHEN Chun-Mei2; SUN Yi1; LIU Guo-Yuan2; YAN Man-Ming1; JIANG Zhi-Yu*,1   

  1. (1 Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200433)
    (2 Institute of Genet-ics, Fudan University, Shanghai 200433)
  • Received:2005-12-23 Revised:2006-05-18 Published:2006-11-28
  • Contact: JIANG Zhi-Yu

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报道了用免疫、电生孔和纳米TiO2光催化组合技术进行杀伤结肠癌LoVo细胞的研究. 先把结合LoVo细胞表面CEA抗原的单克隆抗CEA抗体吸附在纳米TiO2微粒表面, 抗体-纳米TiO2复合微粒就会自动吸附到LoVo细胞的表面, 然后用电脉冲法使LoVo癌细胞的细胞膜上产生小孔, 促使纳米TiO2微粒进入癌细胞内部. 最后在紫外光照射下使TiO2纳米粒子在癌细胞内部发生光催化氧化作用, 杀伤癌细胞. 结果表明, 这种组合技术具有很高的杀癌细胞能力. 在仅含3.12 μg/mL抗体-纳米TiO2的细胞培养液内和强度为4 mW/cm2的紫外光照射下, 可在30 min内将所有LoVo癌细胞杀死. 这种技术对LoVo癌细胞的杀伤力远高于对不表达CEA抗原的人正常TE353.sk细胞的杀伤力, 显示了组合技术杀癌细胞的高选择性. 因此, 这种组合技术有望成为治疗癌症的新方法, 值得进一步探索.

关键词: 人结肠癌LoVo细胞, 人皮肤TE353.sk细胞, 纳米-TiO2免疫, 细胞电生孔, 光催化, 癌症治疗

A new method combined with immunity, electroporation and nano-TiO2 photo-catalysis was successfully used to kill LoVo cancer cells. At first monoclonal antibody was adsorbed on the surface of nano-TiO2 particles, then the antibody-nano-TiO2 conjugates would adsorb on the membrane of LoVo cells spontaneously. Electroporation method was used to produce micro holes on the membrane of LoVo cells, which promoted the entering of antibody-nano-TiO2 conjugates into the cancer cells. Under the irradiation of ultra violet light, the cancer cells could be killed based on the photocatalysis effect of nano-TiO2 particles within cells. The results show that the combined technique is very efficient for killing cancer cells. In the case of using low concentration of antibody-nano-TiO2 as 3.12 μg/mL, and under the irradiation of ultra violet light at 4 mW/cm2, all of the LoVo cancer cells could be killed within 30 min. The photo-killing effi-ciency for LoVo cancer cells was much higher than that for human normal TE353.sk skin cells, indicating the high selectivity of this method. This novel combination method might be used as a new technique for the cancer treatment in the future.

Key words: human colon LoVo cell, TE353.sk cell, nano-TiO2, immunity cell electroporation, photo-ca- talysis, treatment of cancer