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化学学报
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化学学报 ›› 2006, Vol. 64 ›› Issue (13): 1355-1360. 上一篇    下一篇

研究论文

基于碳纳米管和铁氰酸镍纳米颗粒协同作用的葡萄糖生物传感器

王存嫦1,2，阳明辉1，鲁亚霜1，吾国强2，沈国励*,1，俞汝勤1   

  1. (1湖南大学化学化工学院 化学生物传感与计量学国家重点实验室 长沙 410082)
    (2浙江省衢州学院 衢州 324000)
  • 投稿日期:2005-09-14 修回日期:2006-03-16 发布日期:2006-07-14
  • 通讯作者: 沈国励

Glucose Biosensor Based on the Synergy between Carbon Nanotubes and Nickel Hexacyanoferrate Nanoparticles

WANG Cun-Chang1,2, YANG Ming-Hui1, LU Ya-Shuang1, WU Guo-Qiang2, SHEN Guo-Li*,1, YU Ru-Qin1   

  1. (1 State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering,
    Hunan University, Changsha 410082)
    (2 Institute of Quzhou, Quzhou 324000)
  • Received:2005-09-14 Revised:2006-03-16 Published:2006-07-14
  • Contact: SHEN Guo-Li

PDF

900

被引次数

54

将制备的铁氰酸镍纳米颗粒(NiNP)与多壁碳纳米管(CNT)混合, 分散于壳聚糖溶液中, 形成一种新的纳米复合成分(NiNP-CNT-CHIT), 将其修饰在玻碳电极表面. 新复合膜体现了NiNP和CNT之间的协同作用, 由于CNT的良好的传递电子性能, 促使NiNP催化氧化还原能力有了较大的提高. 此NiNP-CNT-CHIT复合膜修饰的玻碳电极在较低电位下对过氧化氢具有良好的电催化性能, 与NiNP-CHIT膜比较, 测定H2O2的灵敏度增大了50倍. 通过戊二醛在电极表面固定葡萄糖氧化酶制备了一种新的葡萄糖传感器. 该传感器在－0.2 V下对葡萄糖的线性范围为0.05～10 mmol/L, 检测下限为10 μmol/L.

关键词: 碳纳米管, 铁氰酸镍纳米颗粒, 协同作用, 葡萄糖氧化酶

Nickel hexacyanoferrate nanoparticles (NiNP) can be easily prepared by mixing hexacyanoferrate and nickel chloride solution at room temperature. The nanoparticles were solubilized in aqueous solution of a biopolymer chitosan (CHIT). With the introduction of carbon nanotubes (CNT), the NiNP-CNT- CHIT system formed shows synergy between CNT and NiNP with the significant improvement of redox activity of NiNP due to the excellent electron-transfer ability of CNT. The NiNP-CNT-CHIT film modified glassy carbon electrode allows low potential detection of hydrogen peroxide with high sensitivity and fast response time. In particular, with the introduction of CNT, it amplified the H2O2 sensitivity by ～50 times compared to film of NiNP-CHIT. With the immobilization of glucose oxidase onto the electrode surface using glutaric dialdehyde, a biosensor that responds sensitively to glucose has been constructed. In pH 6.98 phosphate buffer, interference free determination of glucose has been realized at －0.2 V vs. SCE with a linear range from 0.05 to 10 mmol/L and response time ＜10 s. The detection limit was 10 μmol/L glucose (S/N＝3).

Key words: carbon nanotube, nickel hexacyanoferrate nanoparticle, synergistic effect, glucose oxidase