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化学学报
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化学学报 ›› 1996, Vol. 54 ›› Issue (11): 1089-1093. 上一篇    下一篇

研究论文

稀土元素对腺嘌呤及鸟嘌呤单核苷酸的水解断裂作用

朱兵;李新民;赵大庆;吴亦洁;倪嘉缵   

  1. 中国科学院长春应用化学研究所;中国科学院稀土化学与物理开放实验室
  • 发布日期:1996-11-15

Hydrolysis of aderosine monophosphate and guanosine monophosphate by lanthanides

ZHU BING;LI XINMIN;ZHAO DAQING;WU YIJIE;NI JIAZUAN   

  • Published:1996-11-15

PDF

648

被引次数

25

本文研究稀土元素对5'-腺嘌呤核苷酸(5'-AMP)和5'-鸟嘌呤核苷酸(5'-GMP)的水解断裂作用。空气中CeCl~3在pH 9，37℃能有效地水解断裂5'-AMP及5'-GMP,而其它三价稀土对5'-AMP和5'-GMP的水解断裂作用很小, 铈对5'-AMP的水解断裂速度大于5'-GMP。紫外吸收光谱实验结果表明反应体系中Ce(Ⅲ)部分被氧化成Ce(Ⅳ), Ce(Ⅳ)的氢氧簇合物是使5'-AMP和5'-GMP水解的活性组分。

关键词: 鸟嘌呤, 断裂, 寡核苷酸, 稀土元素, 腺嘌呤, 水解作用, 国家攀登计划基金, 其它基金

The hydrolysis of adenosine-5'-monophosphate (5'-AMP) and guanosine-5'-monophosphate (5'-GMP) by lanthanides was investigated. 5'-AMP and 5'-GMP was efficiently hydrolyzed by cerium(Ⅲ) chloride under air at pH 9 and 37℃, and other lanthanides(Ⅲ) showed less efficiency at the same condition. The hydrolysis rate of 5'-AMP by cerium was greater than that of 5'-GMP. UV spectra showed that Ce(Ⅲ) was oxidized to Ce(Ⅳ) in the reaction mixture. The active species for the hydrolysis of 5'-AMP and 5'-GMP was ascribed to the Ce(Ⅳ) hydroxide cluster in the reaction mixture.

Key words: GUANINE, FRACTURE, OLIGONUCLEOTIDE, RARE ELEMENT, ADENINE, HYDROLYSIS

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