化学学报 ›› 2001, Vol. 59 ›› Issue (5): 746-750. 上一篇    下一篇

研究论文

手性卟啉化合物聚集体与DNA 的相互作用: 电子吸收光谱 和圆二色光谱研究

彭小彬;蔡洁;袁高清   

  1. 华南理工大学应用化学系.广州
  • 发布日期:2001-05-15

The interaction between the assembly of chiral zinc (Ⅱ) prophyrin and DNA: Electron absorption spectrum and CD spectrum studies

Peng Xiaobin;Cai Jie;Yuan Gaoqing   

  1. S China Univ of Polytechnol., Dept of Appl Chem.Guangzhou
  • Published:2001-05-15

使用电子吸收光和圆二色(circulardichroism,CD)光谱研究了手性氨基酸卟啉锌配合物(Thr---TPPZN)聚集体与DNA之间的相互作用,这种螺旋结构的手性卟啉聚集体能与DNA结合,L-Thr----TPPZN聚集体与DNA作用量是通过氨基酸残基与DNA的磷酸链形成氢键,结合模式为外部结合,而D----Thr--TPPZN聚集体与DNA作用除了存在以上这种氢键作用之外,卟啉单元还能部分地插入DNA中,与DNA的碱基对形成π-π堆积作用。L--Thr---TPPZN和D--Thr--TPPZn聚集体与DNA结合模式不同是由于L-------Thr----TPPZn聚集体的左手螺旋结构与DNA的右手螺旋结构不匹配,而右手螺旋结构的D--Thr-----TPPZN聚集体能嵌入同样是右手螺旋结构的DNA中。

关键词: 卟啉, 聚集体, 脱氧核糖核酸, 相互作用, 电子吸收光谱, 圆二色光谱

Using electron absorption spectra and CD( circular dichroism) spectra, we studied the interaction between the assembly of chiral acid modified porphyrinatozinc (Ⅱ)( Thr--TPPZn ) and DNA. The helical assembly of Thr---TPPZn can bind to DNA. The amino acid residue of the assembly of L-----Thr ---TPPZn forms hydrogen bonds with the ohosphate chain of DNA, and the binding belongs to external type. As to the assembly of D----Thr ----TPPZN, the porphyrin unit formsπ-π stacking with the base pair of DNA besides the above mentioned hydrogen bonding. In this case, the binding is a partial intercalation. The reason for the different binding mode is that the assembly of L amino acid modified porphyrinatozinc ( Ⅱ) is left -- handed helical, which cannot match the right --handed helical of DNA, while the assembly of D--amino acid modified porphrinatozinc (Ⅱ) is right --handed helical that can match the helical of DNA.

Key words: PORPHYRIN, AGGREGALES, DEOXYRIBONUCLEIC ACID, INTERACTIONS

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